Hi. I'm interested in using this kit to obtain cytosolic and mitochondrial protein fractions from adherent breast cancer cells and was wondering if anyone had any experience (good or bad!) or advice about it. Thanks.
We have a calculation dilemma for a GH ELISA kit since this kit is designed for volume (liquids) (media or plasma) and we are measuring GH in cell extracts normalized by protein (micrograms of...
07 August 2016 3,186 6 View
We are trying to transfect primary retinal cell cultures (chicken) with a pCAG (Addgene) plasmid but we have very low transfection efficiency (
07 August 2016 9,933 0 View
I am starting an RSM d-optimal design, 5 numeric, 1 categoric variable, but I am a little confused. I don't know if it is strictly necessary to follow RSM protocol and start with a first degree...
02 March 2016 3,898 2 View
I've extracted fractioned protein lysates from adherent breast cancer cells. I've had good results with the cytosolic and mitochondrial fractions. However, the last fractioned obtained is a...
02 March 2016 5,178 4 View
Hi, I'm looking into regulation of mitochondrial apoptosis by a novel compound. Gene expression results suggest it induces upregulation of both BNIP3 and BNIP3L and I'm considering looking at...
02 March 2016 2,244 1 View
Hi, I'm planning to prepare cell lysates to obtain mitochondrial and cytosolic protein fractions from adherent breast cancer cells. I've been looking into abcam's fractionation kit but was...
01 February 2016 4,163 3 View
I have good results showing the induction of PARP cleavage by different agents, but I need to include a positive control for publications. Any suggestions? Thanks!
31 December 2014 5,209 5 View
I've been involved in some analytical electrochemistry experiments to determine the reduction/redox potential of some compounds I'm working with. Cyclic voltammetry of two different analytes has...
10 November 2014 1,354 6 View
I could collaborate by identifying scolopendromorph centipedes (Chilopoda: Scolopendromorpha).
01 January 1970 9,051 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View