Electroporation conditions for primary retinal cell cultures (chick embryo)?

We are trying to transfect primary retinal cell cultures (chicken) with a pCAG (Addgene) plasmid but we have very low transfection efficiency (

07 August 2016 9,853 0 View

Calculation dilemma in a GH ELISA kit designed for media and plasma, Can you help us?

07 August 2016 3,105 6 View

In Response Surface Methodology is it mandatory to always start experimenting with first degree equation? Can one start with a second order one?

02 March 2016 3,798 2 View

What's the best way to shear DNA in a nuclear solution?

I've extracted fractioned protein lysates from adherent breast cancer cells. I've had good results with the cytosolic and mitochondrial fractions. However, the last fractioned obtained is a...

02 March 2016 5,078 4 View

Advice on antibodies for western blot detection of BNIP3/BNIP3L?

Hi, I'm looking into regulation of mitochondrial apoptosis by a novel compound. Gene expression results suggest it induces upregulation of both BNIP3 and BNIP3L and I'm considering looking at...

02 March 2016 2,126 1 View

Suggestions of methods for mitochondrial protein isolation? A good loading control for mitochondrial fraction?

Hi, I'm planning to prepare cell lysates to obtain mitochondrial and cytosolic protein fractions from adherent breast cancer cells. I've been looking into abcam's fractionation kit but was...

01 February 2016 4,090 3 View

What's a suitable positive control for detection of PARP cleavage using western blotting?

I have good results showing the induction of PARP cleavage by different agents, but I need to include a positive control for publications. Any suggestions? Thanks!

31 December 2014 5,135 5 View

Basic electrochemistry question: how does the midpoint potential of an analyte affect its redox reactivity?

I've been involved in some analytical electrochemistry experiments to determine the reduction/redox potential of some compounds I'm working with. Cyclic voltammetry of two different analytes has...

10 November 2014 1,270 6 View

Did you collected centipedes?

I could collaborate by identifying scolopendromorph centipedes (Chilopoda: Scolopendromorpha).

01 January 1970 8,970 2 View

No title

02 March 2021 3,060 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View