Carlos Martínez-Pérez

6 Questions 8 Answers 0 Followers

Questions related from Carlos Martínez-Pérez

Advice on antibodies for western blot detection of BNIP3/BNIP3L?

Hi, I'm looking into regulation of mitochondrial apoptosis by a novel compound. Gene expression results suggest it induces upregulation of both BNIP3 and BNIP3L and I'm considering looking at...

03 March 2016 2,212 1 View

What's the best way to shear DNA in a nuclear solution?

I've extracted fractioned protein lysates from adherent breast cancer cells. I've had good results with the cytosolic and mitochondrial fractions. However, the last fractioned obtained is a...

03 March 2016 5,145 4 View

Has anyone had any experience using the abcam Cell Fractionation Kit (ab109719)?

Hi. I'm interested in using this kit to obtain cytosolic and mitochondrial protein fractions from adherent breast cancer cells and was wondering if anyone had any experience (good or bad!) or...

02 February 2016 1,397 0 View

Suggestions of methods for mitochondrial protein isolation? A good loading control for mitochondrial fraction?

Hi, I'm planning to prepare cell lysates to obtain mitochondrial and cytosolic protein fractions from adherent breast cancer cells. I've been looking into abcam's fractionation kit but was...

02 February 2016 4,139 3 View

What's a suitable positive control for detection of PARP cleavage using western blotting?

I have good results showing the induction of PARP cleavage by different agents, but I need to include a positive control for publications. Any suggestions? Thanks!

01 January 2015 5,187 5 View

Basic electrochemistry question: how does the midpoint potential of an analyte affect its redox reactivity?

I've been involved in some analytical electrochemistry experiments to determine the reduction/redox potential of some compounds I'm working with. Cyclic voltammetry of two different analytes has...

11 November 2014 1,333 6 View