whether GROMACS can do rmsd restrain of a protein it its starting conformation throughout the whole simulation?

Hi all. I have a query. It is about Whether GROMACS can do rmsd restraint of a protein throughout the whole simulation. I found Gromacs can do only position restraint. But my intention is the...

21 November 2020 481 4 View

What is the best Discrete Element Method (DEM) solver, that can be used to for granular packing of super-ellipsoid particles ?

I am interested in packing primary particles defined by super-ellipsoid equations under the influence of particle particle interaction involving friction, torque and reducing particle to particle...

27 October 2020 9,031 1 View

Is it possible to simulate only a particular higher order mode in CST Microwave Studio?

Consider the case of a WR-90 rectangular waveguide (a = 22.86 mm, b = 10.16 mm). If I want to see the fields etc. for say the TE02 mode in CST MWS is it possible to simulate it in particular?...

29 September 2020 8,572 11 View

Where can I find online available current data for Induction Motor or Synchronous Motor stator inter turn fault ?

I am working on the project of developing an algorithm for stator inter-turn failure diagnosis. For that, I am looking for online available data of the current signal from the faulty machine.

08 May 2020 4,667 1 View

How to increase the resistance of low thickness Silane coating against alkali at elevated temperature?

I'm facing a problem when I'm placing one drop of 1% NaOH on my silane coated metal panels and are heating them at higher temperature for example 80 C for 30 minutes or 55 C for 4 hours. The...

05 March 2020 8,332 6 View

Can anyone provide a solution to "severe error message 2070" in Gaussian during perovskite optimization?

Hi All, I have imported a .cif file into Gaussian09 and I am trying to optimize its structure. During the optimization there is an error message "severe error message 2070". When I check the files...

24 November 2019 970 4 View

Microstrip line Characteristic Impedance?

I have 2 questions related to Microstrip Line characteristic impedance: 1) What would be the relation of characteristic impedance of a given microstrip line with frequency? As effective...

09 April 2019 3,728 5 View

Can anyone please solve this question?

A single-phase load is fed through a 66-kV feeder whose impedance is 120+j 400 Q and a 66/6.6 kV transformer whose equivalent impedance (referred to LV) is 0.4 + 1.5 . The load is 250 kW at 0.8...

22 November 2018 8,346 2 View

What is the appropriate methodology to apply shockwave theory at weaving sections?

I want to apply the shockwave theory to the traffic behaviour at weaving section with an auxiliary lane. What is the appropriate methodology? Is it appropriate to apply the theory of moving...

25 March 2018 4,078 4 View

What could be the possible reasons for double drainage samples having larger instant deformation than single drainage samples?

26 November 2017 4,794 2 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View