Saheli Halder

5 Questions 10 Answers 0 Followers

Questions related from Saheli Halder

Has anyone experienced missing nucleotide sequence even without any perturbation?

I have experienced a strange thing while my recent cloning. After I had sequenced my clone i noticed that a part of the nucleotide sequence got missing from the plasmid backbone. I have not done...

10 September 2019 5,098 2 View

Can Nuclepore Track Etch membrane bind and filter out DNA strands?

Can I separate out DNA-protein complex from free DNA by Nulepore Track Etch membrane from Whatman? It is a polycarbonate membrane. Can mixed cellulose membrane be used to bind free DNA?

23 July 2019 3,708 0 View

What is the usual running condition for an Urea PAGE?

Can anyone tell me the usual running conditions (Voltage or power?) for running Urea- PAGE gels? Should the whole gel running apparatus be used in cold room (6deg) or room temperature? I will be...

22 July 2019 9,165 10 View

Can I use XL1 blue competent cells for TopoTA cloning?

We are planning to buy Topo TA cloning kit without the competent cells due to higher cost of the kit. We have home made XL1 blue E.coli chemical competent cells in our lab. We can also prepar some...

15 July 2019 3,997 4 View

Bits vs Nucleotide position graph?

If I have a few oligonucleotide sequences and I want to determine the consensus sequence and put it in a bits vs sequence position graph. How is bits vs nucleotide position graph made? Like the...

03 July 2019 1,739 2 View