Saheli Halder

3 Questions 7 Answers 0 Followers

Questions related from Saheli Halder

Has anyone experienced missing nucleotide sequence even without any perturbation?

I have experienced a strange thing while my recent cloning. After I had sequenced my clone i noticed that a part of the nucleotide sequence got missing from the plasmid backbone. I have not done...

10 September 2019 5,036 2 View

What is the usual running condition for an Urea PAGE?

Can anyone tell me the usual running conditions (Voltage or power?) for running Urea- PAGE gels? Should the whole gel running apparatus be used in cold room (6deg) or room temperature? I will be...

22 July 2019 9,123 10 View

Bits vs Nucleotide position graph?

If I have a few oligonucleotide sequences and I want to determine the consensus sequence and put it in a bits vs sequence position graph. How is bits vs nucleotide position graph made? Like the...

03 July 2019 1,697 2 View