Madison Belk

3 Questions 1 Answers 0 Followers

Questions related from Madison Belk

Decreased LC3A/B output in Westerns with >MOI2 HSV-1?

Has anyone else run Westerns for LC3A/B with HSV-1 infections in human and mouse glial cells and seen little to no LC3A/B expression with viral MOI above 1? I used MOI 1, 2, and 5 a few times and...

25 January 2024 8,194 0 View

Has anyone ever frozen their standard gradient and reused it for subsequent ELISAs and compared results to test if reusing frozen standards works?

I had extra solution from my standard gradient vials after plating my standard gradient in my ELISA wells and was wondering if it was reusable for another hAST IL-8 ELISA? Has anyone seen similar...

19 October 2023 4,942 1 View

Has anyone else seen plastic degradation in the cap of their 1xPBS/0.05% PBS tween 20 storage container?

I was running an ELISA today and noticed there were chunks of plastic from the plastic cap of my 1xPBS/0.05% PBST bottle in the solution. Has anyone else seen this and did it affect your plate washes?

19 October 2023 335 1 View