I need to evaluate viral accumulation by qPCR in plants with DNA and RNA virus coinfections . I dont know how proceed in tge sample treatment before the qPCR . Please someone has done this before ?
Anthropometrically and conceptually, association does not equate causation. However, regarding anthropometric indicators (i.e., metrics of general and abdominal obesity), most of studies worldwide...
20 April 2024 8,779 6 View
I have been culturing human microvascular endothelial cells (HMEC-1) on my own-made low-serum media. The cells expand fine in wells and flasks T25, T75, and T180 using 0.2 ml/cm2, so the media is...
23 November 2023 1,439 1 View
Im running a Gibson Assembly, using two fragments of 2750 pb and a vector of 3300pb. Then when I transform in DH5alfa or 10 beta got a higher band than I expected. Also I did PCR from miniprep and...
03 December 2022 3,356 0 View
I would like to know if is possible to re amplify and PCR fragment for Gibson Assembly ? I did cut and purified the gel band but the amount in ng/uL is not enough for Gibson Assembly later.
22 September 2022 2,102 0 View
I have flow cytometry data of T cells for which I measured the expression of 5 exhaustion markers. I would like to know how many of my cells simultaneously express any combination of 1, 2, 3, 4 or...
17 July 2022 4,584 2 View
I would like to use the SKBR3 cell line in a long term co-culture with engineered T cells. To reduce their proliferative capacity and prevent rapid depletion of the media I would like to irradiate...
06 April 2022 5,533 0 View
I'm designing primers for Gibson Assembly . My main query about that is the harpain and self dimer formation ? Did you have problems with that ?
20 January 2022 2,727 5 View
Dear all, I have recently come across several examples in Social Sciences where supervisors become co-authors of published articles by MSc and PhD students. While I understand a supervisor might...
07 October 2018 2,915 94 View
I am using Chloroquine and its effect on autophagy and I want to treat my cell culture with a dose close to the exposition with CQ treatment. So how valid is to use the mean serum concentration?...
23 November 2017 2,245 2 View
How to establish the appropriate reactions and innteractions between compounds developed in the produce's senescence and colorimetric compounds for the sensor of an intelligent packaging...
14 October 2017 8,587 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View