We're using an NSG mouse, injected with human cells. Following a glucose tolerance test we'll be measuring c-peptide response. Kit says we need 50ul plasma per test, which is "awkward". Any idea of the dilutions we could get away with?
The answer is yes. Many labs did it. Whoever has been implanting human beta-cells or human beta-cell precursors or any other tissue (e.g. hepatocytes) expressing human insulin. My suggestion: Do a pubmed search for mouse (possibly nude or SKID) and insulin and human and (implant or transplant or graft) and choose the right conditions for your experiment.
Hi
You can try the kits either from DRG or Abcam. You can locate them on google.
Wishes,
Bassem
Millipore has an eia that uses 10 ul sample size.
http://www.emdmillipore.com/US/en/product/Human-C-Peptide-ELISA,MM_NF-EZHCP-20K
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