Andrew Bond

4 Questions 3 Answers 0 Followers

Questions related from Andrew Bond

Are there any pig specific antibodies (for IHC) with no cross-reactivity in human tissue?

We are seeding human tissue with pig cells and I am looking for a good antibody to detect the pig cells, with absolutely no cross-reactivity with human cells. Does anyone have experience of any...

02 February 2018 3,515 3 View

What is the best immunostain for human MSCs transplanted under the mouse kidney capsule?

We have co-transplanted human islets with human MSCs under the kidney capsule of NSG mice. Is there a specific stain I can use for hMSCs? Using anti-HLA picks up the human islets as well. Also,...

04 April 2016 6,050 1 View

Has anyone done a human c-peptide assay using mouse plasma, and what sort of dilutions were needed?

We're using an NSG mouse, injected with human cells. Following a glucose tolerance test we'll be measuring c-peptide response. Kit says we need 50ul plasma per test, which is "awkward". Any idea...

12 December 2014 309 3 View

Is there a non-parametric equivalent of a 2-way ANOVA?

I have two groups, drug treated vs control, and obtained tissue and made measurements at 5 different time points. A 2-way ANOVA works for some of the variables which are normally distributed,...

03 March 2014 8,974 98 View