We are using BDNF kit to detect BDNF in rat muscle homogenate.

The standard curve is correct, so the kit is working well, however in our samples have a lot of variability in different concentrations. We can not reproduce the technique with the same samples as it once gives us a concentration value X and the next time gives low values, that it seems that we are not detecting BDNF.

I would be helpful if somebody can give me some information, if you know whether BDNF is degraded so quickly that in some cases can not detect it, whether we should add something special to the homogenization buffer or if I have to process the samples specifically… Thank you!

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