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We want to block and potentiate CB1 receptor in tissue through agonists and antagonist "ex vivo". But how we can check that the drug used is working? We can check by WB the levels of the total...
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I would like to induce injury in skeletal muscle fibers to study muscle regeneration but I would like to preserve nerve terminals intact. Could it be possible? Is there some specific drug? Thank...
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Dear all, My new results show a very clear increase of SOD2 protein levels in muscle tissue. So, I thought that it could be due to an increase of oxidation, that is, an increase of TCA cycle that...
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Hi, I performed differential centrifugation: 3,500 rpm for 15 minutes, and the resulting supernatant was further centrifuged at 45,000 rpm for 1 hour to obtain the cytosolic and microsomal...
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We are using BDNF kit to detect BDNF in rat muscle homogenate. The standard curve is correct, so the kit is working well, however in our samples have a lot of variability in different...
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We want to inhibit the binding of BDNF and NT-4 to its receptor. So we are looking for some peptide inhibitor or antibody that can binds to these proteins in their binding sites. Any suggestion?...
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