Erica Hurtado

8 Questions 18 Answers 0 Followers

Questions related from Erica Hurtado

How to validate agonist and antgonist of CB1 receptor (check CB1 activity) in tissue?

We want to block and potentiate CB1 receptor in tissue through agonists and antagonist "ex vivo". But how we can check that the drug used is working? We can check by WB the levels of the total...

23 April 2024 8,127 0 View

Is there some specific drug to induce injury only in muscle fibers without affecting peripheral nerves?

I would like to induce injury in skeletal muscle fibers to study muscle regeneration but I would like to preserve nerve terminals intact. Could it be possible? Is there some specific drug? Thank...

31 October 2020 9,315 4 View

Increased SOD2 protein in muscle without TCA cycle increased, does it make sense?

Dear all, My new results show a very clear increase of SOD2 protein levels in muscle tissue. So, I thought that it could be due to an increase of oxidation, that is, an increase of TCA cycle that...

11 March 2019 9,718 10 View

How many muscle fibers I have to count to have reliable results?

Hi, I am analysing satellite cells (PAX7 positive) by IHC and comparing the diferences between control vs treatment. To do that, I have to normalize the number of satellite cells to the number...

05 December 2017 7,181 1 View

It is possible to detect (by WB) a membrane receptor, mainly in the cytosolic fraction after a differential centrifugation?

Hi, I performed differential centrifugation: 3,500 rpm for 15 minutes, and the resulting supernatant was further centrifuged at 45,000 rpm for 1 hour to obtain the cytosolic and microsomal...

08 December 2015 5,552 3 View

Has anyone detected BDNF in tissue homogenates by ELISA?

We are using BDNF kit to detect BDNF in rat muscle homogenate. The standard curve is correct, so the kit is working well, however in our samples have a lot of variability in different...

09 January 2015 3,280 7 View

Anyone have suggestions for a peptide inhibitor or antibody that can binds to BDNF or NT-4?

We want to inhibit the binding of BDNF and NT-4 to its receptor. So we are looking for some peptide inhibitor or antibody that can binds to these proteins in their binding sites. Any suggestion?...

01 February 2014 2,614 9 View

Increased mitochondria SOD2 protein in muscle

Dear all, My new results show a very clear increase of SOD2 protein levels in muscle tissue. So, I thought that it could be due to an increase of oxidation, that is, an increase of TCA cycle that...

01 January 1970 2,525 4 View