Is there some specific drug to induce injury only in muscle fibers without affecting peripheral nerves?

I would like to induce injury in skeletal muscle fibers to study muscle regeneration but I would like to preserve nerve terminals intact. Could it be possible? Is there some specific drug? Thank...

30 October 2020 9,077 4 View

Methods for bird trend analysis?

What would be the best method for analysing trends in species abundance in a single area in Latin America over a 15 year period? Could TRIM be used for this purpose? I don't have data about...

27 October 2020 7,532 5 View

How to adapt 280 nm absorbance for protein quantification to microplates ?

Hello. I want to adapt the 280 nm absorbance method for protein quantification of cell lysates to the microplate set up. I learned that for 1 cm quartz cuvettes a conversion factor of 1.3 is used...

06 July 2020 1,818 3 View

How do I extract data Hazard Ratio and CI from Kaplan-Meier curves?

I am conducting a meta-analysis of Hazard Ratios (HR), however, most of the studies presented only Kaplan Meier curves without the HR and Confidence Intervals (CI). I am extracting data manually...

23 June 2020 6,584 1 View

Can 6M Urea inhibit lysozyme action? or this happens the other way around?

I mix both components together with other substances to treat my bacterial pellet and extract protein, but I don't know if Urea 6 M or lysozyme loses its effect

24 April 2020 4,098 14 View

Can someone please share a protocol for the production of recombinant proteins in E.coli BL21 DE3? or the use of plasmid pET 23B?

this for obtein a protein of plasmodium please

24 April 2020 4,302 3 View

How to get forces in a constraint in Abaqus?

Hello everyone! I am doing a static analysis in Abaqus. It's a quite simple model. Two plates connected through to holes using rigid body connections (see attached image). One plate is fixed and...

24 March 2020 3,029 3 View

How can I solubilize HgNO3 in water?

I need to solubilize HgNO3 in water at different concentrations, but I have notice an orange precipitate formation when I dissolved the salt in water. Probably it is mercury oxide. How can I...

28 March 2019 1,277 3 View

Procedure for thawing cryopreserved hpc sample for flow cytometry viability via 7-aad?

Currently our lab performs post thaw viability via trypan blue. We would like to begin using flow cytometry 7-aad for our post thaw viability. Our attempts so far at thawing our samples have led...

22 September 2018 5,396 3 View

How to demonstrate (derivation) that the viscous stress tensor is a linear combination between viscosity and velocity gradient ?

Derivation of stress tensor in terms of viscosity and velocity gradient.

08 September 2018 3,587 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View