Mandy Taisto

3 Questions 4 Answers 0 Followers

Questions related from Mandy Taisto

Why are my spinal cord sections curled after IHCs?

So I'm working with mouse spinal cords and sometimes the sections are curled/rolled after being sectioned/IHC. It doesn't happen to every batch processed but a good portion do. What would cause...

26 February 2015 8,110 5 View

I'm wanting to look at mitochondria within neuromuscular junctions from longitudinal gastroc tissue. Suggestions on how to do so? Protocol? Advice?

We have a transgenic KO mouse model for ALS and are wanting to look at mitochondria as a possible indicator of retrograde transport disruption in motor neurons. We have longitudionally sectioned...

24 April 2014 6,826 4 View

Any suggestions on a hydrophobic barrier pen that isn't destroyed by triton?

I'm attempting to do an IHC on serial sections of muscle using different antibodies on each section. I'm having troubles keeping the liquid from running into adjacent muscle sections during...

19 October 2013 8,953 1 View