Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies?

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Jean-Jacques Pin

There are a large number of clones directed against CD208. But the best clone against DCLamp, recognized by the entire scientific community, is a Rat monoclonal antibody, clone 1010E1 (from Dendritics). this one works perfectly in paraffin, that the biopsy is fixed in Formalin or in Bouin. This clone works on all animal species, and of course on humans. Ref: DDX0191. This clone is regularly used for its good prognostic value in lung cancer of the smoker.

Sorry, I forgot to mention that the clone DDX 0191 (DClamp 1010E1) is available via NOVUS in England

Chris Carey

Jean-Jacques Pin thank you. Yes this is the one I ordered but the anti-rat secondaries don't work in the particular multiplexed project I am trying to optimise. I should have stated this. I really appreciate you taking the time to reply.