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Questions related from Chris Carey
Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies? We have tried several available antibodies for use in human biopsies, but none are...
25 February 2019 6,533 3 View
We are doing some sequential multiplexed IF experiments but are seeing that the emission spectra for the dyes is significantly changing (20-30nm red shift). Thus far we cannot explain it, other...
07 August 2014 207 3 View
Performing multiplexed immunohistochemistry on FFPE tissues. Need to find a reliable and robust green chromogen for membranous staining, one that can be colour-deconvoluted from other colour in...
28 January 2014 2,352 0 View
We are performing multiplexed immunofluorescence on formalin-fixed, paraffin embedded Hodgkin lymphoma. A major issue has been autofluorescence or non-specific binding of what we think are...
20 November 2013 618 2 View
When Hodgkin slides are fixed with DAPI counterfade we are seeing excellent uptake on the small lymphocytes but extremely patchy HRS nuclear staining. Improving this could help with out analysis.
26 July 2013 2,706 4 View