Chris Carey

6 Questions 4 Answers 0 Followers

Questions related from Chris Carey

Is there any database of primary antibodies suitable for conjugation and use on the Hyperion (Fluidgim) imaging platform?

I am struggling to find some antibodies for a project where I am looking at multiplexed targets in human, formalin-fixed & paraffin-embedded tissue. The criteria for suitability...

15 April 2019 2,278 1 View

Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies?

Has anyone been able to optimise DC-LAMP (LAMP3 / CD208) using immunohistochemistry in formalin-fixed biopsies? We have tried several available antibodies for use in human biopsies, but none are...

25 February 2019 6,579 3 View

Does anyone know of the best mounting medium to avoid red-shift in multiplexed Immunofluorescence?

We are doing some sequential multiplexed IF experiments but are seeing that the emission spectra for the dyes is significantly changing (20-30nm red shift). Thus far we cannot explain it, other...

07 August 2014 235 3 View

Anyone know of a reliable and stable green chromogen?

Performing multiplexed immunohistochemistry on FFPE tissues. Need to find a reliable and robust green chromogen for membranous staining, one that can be colour-deconvoluted from other colour in...

28 January 2014 2,383 0 View

How can we minimise / avoid autofluorescence of Eosinophils in Hodgkin tissue?

We are performing multiplexed immunofluorescence on formalin-fixed, paraffin embedded Hodgkin lymphoma. A major issue has been autofluorescence or non-specific binding of what we think are...

20 November 2013 655 2 View

How can I improve the DAPI staining of Hodgkin Reed-Sternberg cells?

When Hodgkin slides are fixed with DAPI counterfade we are seeing excellent uptake on the small lymphocytes but extremely patchy HRS nuclear staining. Improving this could help with out analysis.

26 July 2013 2,740 4 View