Has anyone any experience in using Bovine plasma with human thrombin?

07 July 2016 0 1K Report

Is there any cross reactivity. Can the constituents of one be used with the other despite species difference?

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Starch analysis - Ewers is 10520 method - elevated starch results?

Hi, I am getting elevated results in my starch analysis. It is about 2-3 % higher than usual. We have not changed anything. We are getting the same results over and over. I have checked the...

25 March 2024 1,395 6 View

Functional/non specific disulphide bond formation in proteins?

As an alternative to looking at the conservation of cysteine residues amongst homologs of a protein of interest, is anyone aware of a server that can predict whether disulphide bond formation...

21 March 2024 3,803 1 View

Protein precipitates on purification??

I am trying to purify a his tagged protein using IMAC. The homologs of this protein purify very well. However, this protein precipitates on elution. Buffer composition- 20mM Tris-HCl pH7.4, 500mM...

20 March 2024 9,095 4 View

Multiple bands detected in SDS sample of monomer species (gel filtration fraction)??

I have purified several his tagged proteins using IMAC. To clean up the samples further I ran them through a GF column (Superdex 200 increase 10/30Gl). When I ran an SDS sample containing the...

19 March 2024 4,202 3 View

Colorimetric glycoalkaloids in potato?

Hi, Has anyone got a method to test glycoalkaloids in potato. I don't have an HPLC so I'm looking for a wet chemistry method. I would like to quantify the results. If possible, I want to look a...

13 November 2023 1,668 1 View

Peroxide value and free fatty acid control?

We use an I house sample as a control sample (set up by running a PT sample along side it). However, over a few months it becomes rancid and we have to start the process again. Does anyone know of...

27 July 2023 8,679 0 View

Does anyone know of any research around a recursive learning cycle that promotes contextual learning and flexible thinking in Elementary Education?

Currently exploring similarities around the Design Thinking, Inquiry Learning, and Mathematical Modelling framework.

17 May 2023 8,746 1 View

Best way to inflate the pancreas for mouse islet isolation?

as stated

18 February 2023 7,264 1 View

Binding affinity (KD) of DNA oligos for their complement?

Folks, Does anyone know of a paper showing binding affinity data of DNA oligos for their complement? Ideally it would show data from a 'non-molecular biology method' such as ITC, SPR, or MST. Even...

12 January 2023 2,965 2 View

Can I still use a chi-square/ Fisher's exact test if the following two conditions are not met?

Can you use the chi-squared test if subjects from the groups in the predictor variable can appear multiple times in the response variable categories? & Can you use chi-square if you do not...

21 July 2022 7,061 0 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

I found the zeta potential of exosomes in DLS and it was -8mv. So is it correct or not ?

What should be the zeta potential of bovine milk exosomes

25 July 2024 7,376 5 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

The HCP removal rate is lower than usual in concentration step, why?

Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...

11 July 2024 5,380 0 View

NH3/NF3/H2 Remote plasma Oxi/nit selective etching mechanism help ??

Hello, I am an Etch Engineer. I am performing etching with high T_ox/Nit selectivity using remote plasma. Etching plasma is formed using only NH3/NF3/H2. 1. I wonder if it would be advantageous...

11 July 2024 8,115 2 View