What statistical analysis method should I use with multivariate categorical data?

Hi, I'm struggling quite a bit, Hope someone can help. My research question is roughly to what extent (and what) characteristics of murder and manslaughter cases are related. The data consists...

05 October 2020 3,958 8 View

Problems with Bovine Fibrin Clots?

Hi I am using Fibrinogen (10mg/ml) and Thrombin (1000U/ml) to form fibrin clots. I found that after getting good cross linking initially the clots dissolve to liquid if left in incubator...

26 May 2016 9,416 4 View

When I added the interaction terms, the previously identified significant variables became insignificant?

I'm running a logistic regression test. On my first test, I've identified 6/7 significant explanatory variables. I then ran another logistic regression test with all the interaction terms. The...

05 April 2016 7,655 3 View

Anyone have a good methodology for Agar Gel for adding on top of a cell culture dish (and that allows you to pour/pipette as a thin layer)?

07 February 2016 7,189 1 View

For Fibrin Matrices is >2000 NIH/ml best - or can one use 600-2000NIH/ml in order to produce 1000U/ml concentration (Thrombin from Bovine Plasma)?

- used for making matrices to seed neonatal fibroblasts.

02 February 2016 8,338 1 View

What is the ultimate purpose of Ecology, and what do we hope to achieve in advancing the field?

01 February 2016 9,757 25 View

In what way can spirituality be experientially incorporated into Early Years Education in the UK?

21 May 2015 7,674 4 View

How can I find out the interaction of an osteoarthritis patient's DNA with Vitamin B complex?

I have osteoarthritis patient DNA. Now I want to study the interaction of the DNA with Vitamin B complex. What is the procedure to do so? What types of materials and methods are used to study this...

26 November 2014 1,981 2 View

How can I identify the subtypes of HLAB27 when I used Real-TM PCR which show qualitative results?

I used Real-TM PCR which shows qualitative analysis for identification of HLA B27 after DNA extraction from patient blood sample. The analysis show me whether it is positive or negative. But I...

12 October 2014 3,675 1 View

What is the process of calculating Rf factor from SDS-PAGE after protein extraction?

protein extraction

08 October 2014 7,028 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

How to successfully revive cells stored at -80 degree C for a year?

I am working on C6 cells along with other neuronal cell lines. We have preserved the cells in 90%FBS and 10% DMSO solution. We are reviving the cells after a year and cells are either not binding...

18 February 2021 4,499 1 View

No title

17 February 2021 4,217 3 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View