Rob Staruch

6 Questions 5 Answers 0 Followers

Questions related from Rob Staruch

Extracting RNA from Fibrin Gels?

Hi I am trying to extract RNA from Fibrin gels for qPCR and subsequently RNA sequence. My gels are small volume (250ul). I have had significant issues with getting high values for ng/ul for...

02 August 2019 9,577 2 View

Has anyone any experience in using Bovine plasma with human thrombin?

Is there any cross reactivity. Can the constituents of one be used with the other despite species difference?

07 July 2016 1,321 0 View

Problems with Bovine Fibrin Clots?

Hi I am using Fibrinogen (10mg/ml) and Thrombin (1000U/ml) to form fibrin clots. I found that after getting good cross linking initially the clots dissolve to liquid if left in incubator...

27 May 2016 9,514 4 View

Does anyone have a protocol for lysing fibrin matrices with seeded cells in order to proceed a cell pellet and supernatant on centrifugation?

Fibrin matrices seeded with cells

19 February 2016 1,679 0 View

Anyone have a good methodology for Agar Gel for adding on top of a cell culture dish (and that allows you to pour/pipette as a thin layer)?

For Cell culture/bioreactor. Needs to be relatively quick setting

08 February 2016 7,296 1 View

For Fibrin Matrices is >2000 NIH/ml best - or can one use 600-2000NIH/ml in order to produce 1000U/ml concentration (Thrombin from Bovine Plasma)?

- used for making matrices to seed neonatal fibroblasts.

03 February 2016 8,426 1 View