I have tested my drug on vero cell line as a normal cell line, and not only wasn't the drug toxic for it but also led to the cell growth after a certain concentration,while it had cytotoxic effect on cancer cells. Has anyone had the same experience?
It has happened to me with antitumor molecules. When the concentrations used in these tumor cells, macrophages or normal cells, are not toxic enough they can have a proliferation effect. As I understand it is due to the metabolic response of the cell, to the stress situation that has been exposed
Dear Janny,
Thanks for your response, by saying this, do you mean that you used Vero cell line as a normal cell line?
@janny Villa
is the normal cells is derived from same lineage of cancer cells? It will be important the normal and cancer cells derived from same lineage then cytotoxic data will give conclusive observation
Dear Shahab,
Thanks for your response! Yes, both cell lines are epithelial cells, however vero cells are derived from kidney of green monkey and the cancer cells belong to human colon and mouse colon.
Dear Saba
Thank you for the information. I will use normal colon cells then data will give reasonable comparison as both cells of same lineage. This is what we practice in our lab. Good luck for your study
https://www.atcc.org/products/all/CRL-1831.aspx
This is a normal colon cell line
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