Histological scoring of acute inflammation in Trachea?

Hello, Does anyone have a detailed protocol for H&E histological scoring of acute inflammation in particularly "trachea"? Thank you. Nazli

05 July 2020 7,932 3 View

How can I import 3ddose file which is out put of dosxyznrc to CERR for plotting DVH?

Hi everyone I have some 3ddose files which are the out put of simulation by dosxyznrc and now I am going to draw DVH in CERR. These 3ddose files are read in Matlab and by using CERR ,they will...

30 January 2020 1,514 4 View

How can the " MAXSTACK" or "STACK size " be changed in egsnrc code?

I had faced with an Error ( stack overflow in $SELECT-PHOTON-MFP) while the dosxyznrc was running. I have figured out the Error related to the number of photon splitting and for removing it , the...

11 December 2019 9,458 3 View

How can I attach an amine group (-NH2) to a carboxyl group (-COOH)?

20 September 2019 5,256 5 View

Any advice on how to find type of regulatory interactions between two genes by using any bioinformatics tool or program?

I have list of pair-wise interactions and I would like to find out the type of interaction (gene X inhibits transcription of gene Y or gene A activates transcription of gene B) between them. Is...

31 October 2017 236 2 View

How can you explain the difference between these data ?

Dear researchers, Here comes a puzzling question. I am having difficulty in explaining a difference in the data of two subcontractors in a scientific way. as we all know, polymers properties are...

23 May 2017 3,384 6 View

Can we plot creep master curve of medical devices by using DMA machine ?

16 March 2017 5,665 10 View

Does anyone have the Arrhenius plot of stress vs. time to rupture of HDPE at room temperature ?

Hello researchers, I am looking for the stress vs. time to rupture curve of HDPE @23C in air, however needs be plotted with Arrhenius method and not TTS (time temperature superposition), any help...

06 March 2017 5,069 3 View

Does any body knows if we can get stable transfection out of the PLVX-Tet-on plasmid which was designed originally for lentivirus production?

stable cell line production

18 January 2017 1,916 1 View

Does anyone know why the negative value obtained for figure of merits?

26 June 2016 3,281 6 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View