has anybody used pronase digestion on the cell culture media? if yes, could you please provide me a brief protocol?
Hi
I use pronase digestion to extract chondrocytes from cartilage.
Preparation of pronase: 500mg in 500ml DMEM (10% FBS + 1Ab), Filter with a 0.2μm, then aliquots in 25ml.
I treat my cartilage with 25 ml of pronase for 1H in a 37 ° C shaker.
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