Which DL architecture is better to work on handwritten data?

I am working in the area of Handwriting recognition for Bangla. As there are many DL architecture, I am confused which one to select.

15 February 2021 4,996 1 View

Do you have any experience of taking astaxanthin supplement?

Astaxanthin is a keto-carotenoids having reddish color. It occurs naturally in certain algae and causes the pink or red color of different seafood especially salmon, shrimp, trout, and lobster....

29 January 2021 7,861 2 View

How to remove/reduce Back Reflection from Objective?

I want to have a optical system where, I focus a beam onto the BFP of a microscope objective. Unfortunately, I always have a strong Back Reflection from the objective. Can anyone suggest me some...

18 January 2021 2,062 8 View

How to calculate binding energy and different interactions from a pdb file of protein-ligand complex?

I have done a docking study by using mcule.inc web-service. I got the .pdb file of my protein-ligand complex from there. As I am using a free student account, I don't have access to more...

14 January 2021 2,321 2 View

Why there is no increase in virus titer after 24 hour of seeding for harvest?

I am working on virus production through bioreactor,my concern is regarding ,no increase in virus titer after 24 hour of seeding for harvest Usually we seed the fermenter and incubate for 72...

05 January 2021 3,860 2 View

Does anyone have experience using High Molecular Weight DNA Extraction Kits for extracting DNA from plant leaves?

Hi there, I'm looking for a High Molecular Plant DNA Extraction Kit for subsequent Nanopore sequencing. Does anyone have experience using commercially available kits? Any...

03 January 2021 9,487 4 View

How to optimise or tune the hyperparameters of Gaussian Process Regression?

I am working with the algorithm Gaussian Process Regression. I used gaussian process regression module from scikit learn library of python. But I couldn't correlate the hyperparameters of Gaussian...

24 December 2020 4,864 4 View

Can you suggest me how can I use D2O for dissolving chitosan sample for NMR analysis?

I am trying to dissolve chitosan with pure D2O but it is not dissolving. I read some articles that used D2O for dissolving chitosan before 1H and 13C NMR analysis? Do I need to do some...

14 December 2020 6,931 5 View

How many chapters (objectives) are necessary for a standard Ph.D. thesis?

This question is focused on biological, chemical, and material science. I think the answers also will help the future Ph.D. students for writing a good Ph.D. research proposal.

10 December 2020 2,090 34 View

Running a Kruskal-Wallis test on a large sample with a likert scale on excel?

Hi everyone, I would like to run a Kruskal-Wallis test on a large sample with a likert scale. I am having difficulties figuring out how to process with the ''rank_avg'', and rank sum with such a...

05 December 2020 793 3 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View