Has anoyone injected human PBMCs in SCID mice?

More Mallu Abhiram Charan Tej's questions See All

Is there a database that can give out cell specific gene expression from an input gene list?

I am interested in finding a database where I can input a list of genes or gene IDs and get the information about how many and which of these genes are largely expressed in a particular cell type....

09 October 2018 535 3 View

Can someone please look at it and tell me if it would be worthwhile pursuing this sample for hybridization for microarray?

Is it prudent to use an RNA sample which has no RIN number, when analyzed using a Bio-analyzer (Agilent). The profile shows a good 18S peak. However, the 28S peak is very small.(probably the...

06 July 2015 9,881 8 View

Can we anesthetize mice before giving oral gavage?

I am working with C57BL6 mice, which are little aggressive. I need to feed them by oral gavage. We have tried doing it in wake animals but they are giving us a tough time holding them and even if...

04 May 2015 8,720 11 View

Is anyone familiar with rosuvastatin in cell culture applications?

What is the ideal solvent and concentration in which rosuvastatin-calcium can be dissolved, for cell-culture applications? Is it soluble in water or do we need ethanol or DMSO solutions? If so...

09 October 2014 5,360 3 View

Can anyone help with Drabkin's assay standard hemoglobin solution and in vivo matrigel plug assay?

Can human hemoglobin be used to prepare the standard curve for Drabkin's assay to estimate the hemoglobin concentration in the matrigel plug injected in mice?

01 February 2014 4,940 0 View

Can someone advise on a storage of isolated PBMCs for microarray or NXG sequencing?

I want to store the isolated PBMCs of my study subjects in the best possible way that their RNA is preserved and in pristine state for microarray/NXG sequencing analysis. I tried snap freezing the...

31 December 2013 4,871 2 View

Redissolution of BSA after salting out with ammonium chloride?

Is it a common phenomenon that BSA redissolves after being salted out by ammonium sulphate upon increasing the concentration further? If so what could be the reason for it?

08 September 2013 1,314 0 View

Simultaneous analysis of cell surface marker expression and cell cycle analysis in PBMCs?

How to analyse freshly isolated peripheral blood mono nuclear cells simultaneously for CD34 expression and the cell cycle phase they are in. The options I have is to use propidium iodide or...

08 September 2013 6,462 2 View

Can someone suggest a protocol for proper detection of the nitrites using Griess assay?

I am trying to measure nitrite content in serum samples by Griess assay. But I am facing problems like curdling of serum (possibly due to plasma contamination). Do I have to do some pre-treatment...

07 August 2013 2,074 1 View

What are the markers for which staining has to be done to enumerate the rodent haematopoietic stem cells from peripheral blood?

Rat / mouse haematopoietic stem cell enumeration from peripheral blood (retro orbital bleeding) by flow cytometry. Please suggest any protocol or kits if available. Thank you.

07 August 2013 7,383 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Baptiste Lamarthée

Several models of xenogeneic graft habe been developped but generally they use NSG or NOG (NOD SCID Gamma-/-) mice and not only SCID.

Personnally, I'm using NSG mice and I inject 30 millions of PBMCs intravenous in Sterile PBS. The mice develop graft versus host disease. I attached a reference talking about it.

Article Human peripheral blood leucocyte non‐obese diabetic‐severe c...