Hansenula polymorpha (Pichia angusta) heat shock transformation method?

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Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

Can sulfuric acid be used to prepare anthrone reagent when it has oxidized and changed color in reaction to light?

Sulfuric acid has changed color to yellow.

16 July 2024 6,964 0 View

What is the suitable freezing media and protocol for human mature adipocytes cryopreservation?

I used regular freezing media (90% FBS and 10% DMSO) for cryopreservation of isolated human mature adipocytes from adipose tissue in -80 degree. When I freezed the cells, the number of cell was...

26 May 2024 7,803 0 View

How it is possible to analyze human mature adipocytes cell markers with flow cytometry? what are the essential optimizations?

As human adipocytes can be up to 300 μm in diameter, How these cells can be analyzed for cell markers by flow cytometry with 100 micron Nozzles? What changes are necessary to be done on flow...

12 May 2024 2,254 0 View

Gel Electrophoresis Troubleshooting?

Hello everyone, in the gel results image provided, the samples in lanes 2 and 5 represent linearized plasmid DNA from groups A and B respectively. Additionally, the samples in lanes 3 and 6 depict...

25 April 2024 7,126 1 View

How can i remove oil layer from adipocyte layer in human mature adipocyte isolation?

I want to isolate adipocytes from human adipose tissue. but after isolation and culture them, there were very larg droplets between small to moderate size cells in 12-wells plate that seemingly...

20 March 2024 5,945 0 View

I want to adhere mature human adipocytes to bottom of Petri dishes or flasks for cell culture. Which coated material or method do you recommend?

After mature adipocytes isolation from human adipose tissue, I want to adhere them for my studies (Adipocytes tend to suspend on top layer of the media). Which method or materials do you...

06 March 2024 904 1 View

How can I establish a bond (amine and carboxylic group) between plga and peg?

Hello, how can I establish a bond (amine and carboxylic group) between PLGA and PEG as a drug carrier? What is the problem with my work? please guide me The mixture containing PEG,...

18 January 2024 9,737 0 View

Why does the coercivity of cobalt ferrite decrease at low temperature 70k?

Following diagram show Hysteresis loops of CoFe2O4 Spherical nanoparticles at 300k & 70K (liquid Nitrogen). Why such decrease in hysteresis loop and Hc have been seen with reduce temperature?

13 August 2023 1,275 5 View

How to remove lime deposited on pottery without ruining pottery and painted motives on it?

I have some pottery sherds with significant motives on them, but unfortunately, lime covered most of them, is there any way to remove the lime without damaging the pottery surface or painted motives?

24 July 2023 2,986 0 View

Problems about acousto-optic coupling in Comsol, how to check whether the moving mesh is correct?

I’m trying to reproduce the acousto-optic coupling simulation in the paper Frequency–angular resolving LiDAR using chip-scale acousto-optic beam steering. LNOI(lithium niobate on insulator) is...

13 August 2024 9,795 0 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to heat polymer for about 130° without oil bath ?

is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it

06 August 2024 947 1 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Where can I find free research instruments for Nursing?

for eg. transition shock scale

01 August 2024 5,998 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

What are the perfect mathematical model for a solar-powered ship implementation?

A computational study of heat flux on cylindrical surfaces using CNT hybrid nanofluids

28 July 2024 4,985 1 View

Seeking recommendations for equipment to mimic sunlight and heat up a greenhouse system?m?

I would like to conduct artificial sunlight experiments to mimic heat capture and air warming in a greenhouse system. The main objective is to heat up the temperature inside the greenhouse, not...

28 July 2024 4,006 2 View

Can anyone kindly suggest research topics in the area of thermodynamics, heat transfer, fluid mechanics or renewable energy?

I am an undergraduate mechanical engineering student seeking suggestions on topics related to either thermodynamics, fluid mechanics, heat transfer or renewable energy for my final year project....

24 July 2024 4,058 5 View