I am going to start work with Bacillus subtilis.
I want to use the Brain Heart Fusion and Nutrient Broth for culture at 37c, 240rpm for 24 hours.
Is the plan quite okay, can anyone suggest me about that?
Dear Shamsul,
we use LB (Luria Bertani) medium with 2 g/L glucose or M9 minimal medium to cultivate B. subtilis.
Have a look: http://subtiwiki.uni-goettingen.de/wiki/index.php/M9_minimal_medium
Good luck
Michael
Dear Shamsul,
Brain Heart Fusion broth is selective for Streptococci. So , Nutrient broth works good for your startup experiments or LB (Luria Bertani) medium . I suggest 150 rpm. Based on your target product you can optimize the media components and growth conditions for maximum production.
All the best
Thank you everyone,
Thank you all for your kind anticipation.
I applied three kind of media for the culture YPD, Brain Heart Infusion, LB and NA (solid media). I find out YPD and LB in 37c 175rpm for 20h-24h the cell growth is much high then the Brain Heart Infusion.
But until I didn't count the cell through the spectrophotometer. I will do it very soon
Thank you
I was tried at 37c temperature but it was excessive growth over night... I will suggest maintaining the temperature between 30-35c. over night.
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