Dear Shamsul,

we use LB (Luria Bertani) medium with 2 g/L glucose or M9 minimal medium to cultivate B. subtilis.

Have a look: http://subtiwiki.uni-goettingen.de/wiki/index.php/M9_minimal_medium

Good luck

Michael

Dear Shamsul,

Brain Heart Fusion  broth is selective for Streptococci. So , Nutrient broth works good for your startup experiments or LB (Luria Bertani) medium . I suggest 150 rpm. Based on your target product you can optimize the media components and growth conditions for maximum production.

All the best

Thank you everyone,

Thank you all for your kind anticipation. 

I applied three kind of media for the culture YPD, Brain Heart Infusion, LB and NA (solid media). I find out YPD and LB in 37c 175rpm for 20h-24h the cell growth is much high then the Brain Heart Infusion.

But until I didn't count the cell through the spectrophotometer.  I will do it very soon

Thank you 

I was tried at 37c temperature but it was excessive growth over night... I will suggest maintaining the temperature between 30-35c. over night.