How to check secretory protein concentration from cell culture media by spectrophotometer?

I am going to culture a cell line with 20% FBS in IMDM medium. Those cell will secrete protein. I need to check the protein concentration from the culture medium by using spectrophotometer. Can...

08 September 2019 8,494 1 View

How to prepare bacterial complex of several different strain of bacterial mixture to treat mouse.??

I want to treat a bacterial mixture to mice. I have 6 different strain, now how should I prepare the bacterial complex mixture to mouse.

05 June 2018 8,801 2 View

RT-PCR suggestion??

I am doing RT-PCR from human blood sample with the hBeta actin. But the bands (in different groups) showing different expression. some are high some r low. In case of beta actin it should...

07 August 2016 7,545 1 View

Mouse Erap1 primer?

I want to work on mouse Erap1 gene. I design the primer but its not specific and I couldn't find any reference for the mouse Erap1 primer. Can any one help me out please!!!!

06 July 2016 3,669 2 View

Erap1 expression by FACS analysis?

06 July 2016 8,143 1 View

Growth condition of Bacillus subtilis in liquid culture?

04 May 2016 5,437 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View