S M Shamsul Islam

7 Questions 18 Answers 0 Followers

Questions related from S M Shamsul Islam

How to check secretory protein concentration from cell culture media by spectrophotometer?

I am going to culture a cell line with 20% FBS in IMDM medium. Those cell will secrete protein. I need to check the protein concentration from the culture medium by using spectrophotometer. Can...

09 September 2019 8,589 1 View

How to prepare bacterial complex of several different strain of bacterial mixture to treat mouse.??

I want to treat a bacterial mixture to mice. I have 6 different strain, now how should I prepare the bacterial complex mixture to mouse.

06 June 2018 8,917 2 View

RT-PCR suggestion??

I am doing RT-PCR from human blood sample with the hBeta actin. But the bands (in different groups) showing different expression. some are high some r low. In case of beta actin it should...

08 August 2016 7,633 1 View

Mouse Erap1 primer?

I want to work on mouse Erap1 gene. I design the primer but its not specific and I couldn't find any reference for the mouse Erap1 primer. Can any one help me out please!!!!

07 July 2016 3,746 2 View

Erap1 expression by FACS analysis?

Is it possible to get the expression of Erap1 through the FACS analysis?

07 July 2016 8,225 1 View

Growth condition of Bacillus subtilis in liquid culture?

I am going to start work with Bacillus subtilis. I want to use the Brain Heart Fusion and Nutrient Broth for culture at 37c, 240rpm for 24 hours. Is the plan quite okay, can anyone suggest me...

05 May 2016 5,540 4 View

Quercetin, How much I should administrate to mice?

For quercetin how much I should administrate to mice, and what will be the administration route? Please suggest

05 May 2016 620 2 View