Is it possible to get the expression of Erap1 through the FACS analysis?
Through the specific primary antibody and conjugated secondary antibody Erap1 detection is possible through FACS analysis.
I am going to culture a cell line with 20% FBS in IMDM medium. Those cell will secrete protein. I need to check the protein concentration from the culture medium by using spectrophotometer. Can...
08 September 2019 8,615 1 View
I want to treat a bacterial mixture to mice. I have 6 different strain, now how should I prepare the bacterial complex mixture to mouse.
05 June 2018 8,948 2 View
I am doing RT-PCR from human blood sample with the hBeta actin. But the bands (in different groups) showing different expression. some are high some r low. In case of beta actin it should...
07 August 2016 7,668 1 View
I want to work on mouse Erap1 gene. I design the primer but its not specific and I couldn't find any reference for the mouse Erap1 primer. Can any one help me out please!!!!
06 July 2016 3,782 2 View
I am going to start work with Bacillus subtilis. I want to use the Brain Heart Fusion and Nutrient Broth for culture at 37c, 240rpm for 24 hours. Is the plan quite okay, can anyone suggest me...
04 May 2016 5,567 4 View
For quercetin how much I should administrate to mice, and what will be the administration route? Please suggest
04 May 2016 660 2 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.
07 August 2024 5,307 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View