Many papers and standard lab protocols describe growing Agrobacterium at 28 degrees. However, few papers also describe doing the same at 29 degrees, and some protocols give a freedom of 28-30 degrees. Again, researchers have reported plasmid cure and deleterious effect in Agrobacterium above 30 degrees (heat-shock like effect). How about growing Agrobacterium at 29 and 30 degrees compared to widely used 28 according to your experiences? Any significant changes? Any advantages at 29 or 30? I'm talking about growing temperature for growing Agrobacterium overnight just before infection, and not during co-cultivation.
We use 30C as it was the temperature recommended by manufacturer where we bought the Agrobacterium, we did'nt find any difference between growing at 29C and 30C.
Temperature Effects on Agrobacterium Phytochrome Agp1
October 2011PLoS ONE 6(10):e25977
DOI: 10.1371/journal.pone.0025977
- Badges
- Science topic