Please can anyone recommend a good book on the study of enzymes (from scratch to purification and analytic techniques) for someone who is new to enzyme studies? Thanks in anticipation.
Enzymology by Price and Steivens
and Introductory enzymology by T. Palmer are two sufficient books in itself
After you get your purified enzymes, you´ll need to characterize them. To understand enzyme mechanisms and enzyme catalysis, try to look:
- The Organic Chemistry of Enzyme-catalysed reactions (http://books.google.com.br/books/about/The_Organic_Chemistry_of_Enzyme_Catalyze.html?id=toy5IMAVswkC&redir_esc=y)
and
- Enzyme Kinetics - a modern approach
(http://books.google.com.br/books/about/Enzyme_Kinetics.html?id=dkmishhU3jAC&redir_esc=y)
Hamada and Rodrigo, pls can you kindly send the books to my email [email address deleted]? I have been unable to download them from the links you provided. Thanks
Protein Purification: Principles and Practice by Robert Scopes is a great book to read for someone new to protein chemistry. Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis by Robert Copeland and Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems by Irwin H. Segel are great for understanding enzyme behavior and how to analyze data.
The books are available for purchase from Amazon.com. You might be able to search google for free downloadable copies of each, but I'm not sure since I have hard copies.
I've recently bought the Copeland and the Segel from Amazon.com. Both of them are great books for enzymology!
Enzymology by Price and Steivens
and Introductory enzymology by T. Palmer are two sufficient books in itself
Hello, All the books for protein purification (...) have been mentionned. For enzyme kinetics, I'll go with the Fersht, also previously mentionned but not with the Segel. In this last book, Segel developped all kind of mechanisms (some of them might not even exist) and people might be tempted to pick up an equation without understanding what's behind. Also, it is quite heavy to read. So, the Fehrst. Or there is an excellent book from Cornish-Bowden: Fundamental of enzyme kinetics. http://www.amazon.com/Fundamentals-Enzyme-Kinetics-Athel-Cornish-Bowden/dp/3527330747/ref=la_B001H9Y5V8_1_1?ie=UTF8&qid=1347312156&sr=1-1
I recomend you this one,
M. Dixon, E. C. Webb, C.J.R Thorne and K.F. Tipton (1979) Enzymes. 3rd Edition, Longmans, Green & Co., London, and Academic Press, New York.
It may be quite old, but it is fantastic, it is a classic in the genera.
Unfortunately all of these books are fundamentally flawed as they continue to spread the classical inhibition equations which do not distinguish between Ki which is a binding equilibrium term and the actual affect the binding has on the enzyme.
The term 1+i/Ki is clearly an equilibrium binding term between inhibitor and the enzyme and there is clearly no information provided by this term as the the change the binding causes to the enzymatic activity.
I think all important books have been mentioned. I recommend both books suggested by Vinod Kumar. They are very easy to follow and understand.
How do i get a good book of enzymology that treat transient state kinetics
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Enzymology