Is there a simple biochemical assay for lytic polysaccharide monooxygenases (LPMO)?

I need a simple protocol for the assay of LPMO enzymes using biochemical assays just like those of cellulases. Most of those I have seen in the literature used mass spectrometry. I would be...

06 July 2017 8,389 3 View

How should negative OD readings be processed in DNS assay for reducing sugar?

Sometimes I get negative OD readings (at 540 nm) when determining reducing sugars using glucose as standard (Miller, 1959). When I use Microsoft Excel to plot the glucose standard curve, the line...

03 April 2015 4,525 15 View

Why does the specific activity of my enzyme decrease at each step of purification?

Can anyone provide possible reasons why the specific activity of my cellulase (from bacteria) keeps reducing after each step of purification? So far, the crude enzyme has the highest activity...

11 December 2014 8,944 34 View

Why is enzyme not precipitating even at 90% ammonium sulphate saturation?

I am trying to precipitate a bacterial cellulase produced on a lignocellulosic hydrolysate as the first step in the purification of the enzyme. I did step-wise ammonium sulphate fractionation at...

07 August 2014 4,331 18 View

What is the most suitable column to analyze the composition of lignocellulosic hydrolysate using LCMS?

The use of HPLC might give some unknown peaks. However, LCMS would allow a more detailed identification of the compounds in the hydrolysate. Such compounds might include sugars (monomers and...

07 August 2014 5,385 4 View

Is it compulsory to deduct OD readings of enzyme blanks in cellulase assays?

One of the most cited references regarding cellulase (saccharifying cellulase, endo-, exo- and betaglucosidase) assays (Ghose, 1987 & Wood and Bhat, 1988) recommend that OD readings of enzyme...

03 April 2014 6,141 1 View

Conflict between BIOLOG and 16S rRNA sequence BLAST in identifying a bacterial strain?

A bacterium was identified by BIOLOG as a particular species; running a BLAST search on the 16S rRNA sequence of the same organism gave a different specie of the same genus. The one with the...

31 December 2013 1,706 28 View

Can someone advise on the enzyme concentration to release 2 mg glucose in cellulase filter paper assay?

The IUPAC procedure for determining total cellulase activity using filter paper states that one should prepare two dilutions of the enzyme such that one dilution will release > 2 mg glucose from...

10 November 2013 3,109 5 View

Does keeping of broth samples at -20degC affect cfu, OD and enzyme activity readings?

In running an experiment to plot growth curve of an organism which is growing on a cellulosic substrate, it is necessary to collect samples from the shake flask at intervals (2-4 hours over 72...

06 July 2013 7,926 10 View

Tips on Writing Review Article

What are the important guidelines for one who wishes to write a review article for publication in a reputable journal for the first time?

03 April 2013 4,567 13 View

No title

02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View

By which mechanism does potassium hyrdoxide works as an activator in the preparation of activated carbon?

Activated carbon is prepared from biomass using KOH as the activating agent. What is the possible reaction between KOH and the biomass?

14 February 2021 8,095 4 View