I need a simple protocol for the assay of LPMO enzymes using biochemical assays just like those of cellulases. Most of those I have seen in the literature used mass spectrometry. I would be grateful if anyone can help.
This article may have what you are looking for, as well as references to other assay formats that you might try.
https://www.researchgate.net/publication/307551775_Overproduction_and_Characterization_of_a_Lytic_Polysaccharide_Monooxygenase_in_Bacillus_subtilis_Using_an_Assay_Based_on_Ascorbate_Consumption
Article Overproduction and Characterization of a Lytic Polysaccharid...
Article A fast and sensitive activity assay for lytic polysaccharide...
The assay is working like a standard peroxidase or laccase assay. You only need a photometer and the solutions.
Thanks for the responses.
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