How do I find out if a Journal is indexed in Scopus, Scimago or Thompson Reuters?

How do I find out if a Journal I want to publish with is indexed in Scopus, Scimago or Thompson Reuters?

10 October 2020 4,515 4 View

What's wrong with my culture of NK92 cells?

Hi everyone, I am trying to culture IL-2 independent NK-92 cell line for weeks now but they are still not proliferating. Media used: MEM with 10% NU serum, 0.2 mM myo-inositol, 0.02 mM folic...

27 September 2020 2,564 6 View

Number of Estimating Equations Required to Test Hypotheses?

After literature review, current draft as below: Variables: Independent = B (represented by I, M, O, S, C), Moderating = W Dependent = F Control = CV Hypotheses: H1a. I has significant...

18 September 2020 7,055 2 View

Which antibodies are recommended for CTLA-4, Tigit, Lag-3 and Tim-3?

I am new to immune oncology. What are the best antibodies against CTLA-4, Tigit, Lag-3 and Tim-3 for IHC and Flow cytometry? Are there any problems I need to be aware of?

19 August 2020 6,258 4 View

Immobilizing an antibody with Protein A/G for SPR?

I am planning to perform an antibody-ligand interaction study with SPR/Biacore. The idea is to immobilize my antibody with Protein A/G. It would be great if you could share your comments, what to...

23 June 2020 7,705 3 View

What is the difference between Introduction and Background in a manuscript?

Some journals do require that a manuscript contain Introduction as a heading as well as background as another heading. This clear separation make it difficult for distinguishing what information...

08 March 2020 7,479 11 View

How do I compare regression coefficients in terms of effect size?

Hi everyone, I have two distinct samples, and in both samples I am examining the relation between age and income. I therefore conduct simple regression analyses (where y represents income and x...

18 February 2020 3,763 3 View

Recommendation for battery holder (coin cell and pouch cell) for electrochemical impedance spectroscopy (EIS)?

Can someone recommend any battery holder (coin cells and pouch cells), which is suitable to perform electrochemical impedance spectroscopy? Especially shifting contact resistances appear to be a...

11 February 2020 192 1 View

How to fixate particles with non-luminescent gelantine subsitute for UV light?

I would like to fixate some nanoparticles in a glass cuvette and excite them with UV (355nm and 266nm) laser light, studying their luminescent properties. I tried gelantine, but this is...

19 November 2019 8,081 4 View

How to analyze EEG data from Smarting (xdf) with Fieldtrip?

Dear all, I would like to analyze my Smarting EEG data (xdf) with Fieldtrip. Has anyone ever done that and can give me some tips? Unfortunately I have not found a function for the import of...

18 November 2019 2,403 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View