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Hello, I want to generate bone marrow derived DCs (BMDCs) and want to use supernatant of a GM-CSF producing cell line for the GM-CSF source. Does someone know a cell line (mouse) which is...
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Hey, I do have a question with murine GM-CSF and M-CSF for making rat BMDCs and BMDMs? I want to use WEHI 3B and L929 conditioned cell culture media in order to generate rat BMDCs and BMDMs. Is...
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Hi, Does anybody know how much of human blood I will need in order to get monocytes derived monocytes? I saw some protocols which use 300ml. That is a lot of blood and I`m not sure, if we will get...
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