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Questions related from Ana Ramon Vazquez
We bought Dynabeads from Invitrogen attached to protein A to perform ChIP assays. The reason is that apparently they were easier to work with than the agarose ones and suitable for sequencing as...
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I have used the substrate "ABTS" (Sigma) which needs a "wavelenght correction set at 650nm", as specified on the Data sheet. I think the plate reader (BioRad) is quite old and I haven´t been able...
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The supernatants are from thioglycollate elicited peritoneal macrophages treated with LPS, or LPS and ATP.
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