Ana Ramon Vazquez

3 Questions 5 Answers 0 Followers

Questions related from Ana Ramon Vazquez

Why are my Dynabeads coagulating during washes in ChIP?

We bought Dynabeads from Invitrogen attached to protein A to perform ChIP assays. The reason is that apparently they were easier to work with than the agarose ones and suitable for sequencing as...

23 April 2015 4,574 11 View

How can I calculate concentrations for cytokines from my data obtained in an ELISA assay if my plate reader doesn't have "wavelength correction"?

I have used the substrate "ABTS" (Sigma) which needs a "wavelenght correction set at 650nm", as specified on the Data sheet. I think the plate reader (BioRad) is quite old and I haven´t been able...

29 April 2013 7,272 1 View

Can I concentrate supernatants for ELISA tests using a centrifugal filter device?

The supernatants are from thioglycollate elicited peritoneal macrophages treated with LPS, or LPS and ATP.

11 April 2013 8,795 2 View