How to do FE analyse of aluminium and steel diffusion bonding?

Finite element simulation of diffusing bonding of steel and aluminium subjected to temp of 500 0c and load of 15tonne in vacuum.

03 March 2020 1,347 2 View

Suitable human neuronal cell line for neurotoxicity assay?

I'm looking for a suitable human neuronal cell line for neurotoxicity or neuroprotection screening. Which human neuronal cell model is best for high throughput screening? Can anyone help?

30 September 2015 7,997 3 View

Which is the best available NOC (Network on Chip) simulator?

I know there are some simulators available, but which has the best accuracy, i.e. great correlation with HDL results?

22 February 2015 5,436 12 View

Why some people says that "energy or power dissipation" can't be considered as performance metrics for a digital circuit?

15 November 2014 1,580 4 View

How can we understand ion beam induced elongation of metal nanoparticles (embedded in insulating matrix) with size smaller than ion track size?

Ion beam induced deformation (elongation along ion track) of metal nanoparticles embedded in silica has been observed, if the size of nanoparticles exceeds the ion track size. Does that mean there...

06 April 2014 5,823 9 View

What is the importance of surface wettability on SERS based ultrasensitive detection of biomolecules?

Among superhydrophobic or superhydrophilic substrates which one is better for SERS-based ultrasensitive detection of aqueous biomolecules and why? If a substrate is superhydrophobic, can it be...

05 April 2014 2,028 3 View

How to fit the Nyquist impedance plot for obtaining the bulk resistance of a polymer film?

In general, the intersection of the high frequency side semicircle to the real axis gives the bulk resistance. This is quite easy, if the center of the semicircle lies on the real axis. But in...

22 January 2014 5,745 4 View

What is the best way to check RNA integrity apart from running a gel?

Sometimes after RNA isolation when it's quantified the concentration seems to be very good (>300ng/ul), however, often the RNA is of very poor quality. Its integrity has been suffered. So what...

03 November 2013 1,307 8 View

No title

02 March 2021 3,060 3 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View