Glutamatergic or GABAergic or dual phenotype neurons ?

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How to evaluate the crystallinity density and amorphous density of a polymer blends by using dsc thermogram ?

Xc= (&-&a)/(&c-&a) and we have Xc 32% and &=0.91g/cc calculate the &a (amourphous density )= ? &c (crystalline density of polymer)= ?

19 January 2023 2,973 3 View

Is there anybody who has STATA or R do file to calculate Buy and Hold Abnormal Return (BHAR) and Calendar Time Portfolio Return(CTPR)?

Both BHAR and CTPR are standard methodology widely followed to measure the long run firm performance.

21 December 2022 8,822 0 View

What is an appropriate measure of multivariate function bias towards any of its input variables ?

This is in context of the objective function of a multivariate optimization problem say, f(a,b,c). I am looking for a "measure" for the degree of bias of f(a,b,c) towards any of the input variables.

15 November 2022 7,522 4 View

Can anyone help me with a company/vendor name who does whole proteomic studies (either by iTRAQ or TMT) in India?

I need to do the proteomic profiling of some of my experimental samples. Please let me know if anyone has the contact number or email ID of such service providers. Thank you!

20 October 2022 10,023 0 View

How to do FE analyse of aluminium and steel diffusion bonding?

Finite element simulation of diffusing bonding of steel and aluminium subjected to temp of 500 0c and load of 15tonne in vacuum.

03 March 2020 1,511 2 View

Does gaussian ignore imaginary frequency while calculating thermochemical properties of transition state?

Transition state from Gaussian QST3 must have imaginary frequency. For zero point energy correction if someone takes this frequency then it'll be erroneous. My concern is that whether Gaussian 16...

25 August 2019 2,464 2 View

Can anyone suggest modifications in this circuit intended for power supply ripple filtering ?

Hi, I have a design for source-follower low pass filter which I intend to use with a switched mode power supply. I simulated the circuit in NI Multisim, and for square wave ripple voltage of...

03 January 2019 3,261 9 View

How to model diffusion bonding of impeller vane by FEA ?

literature, FE modelling / analysis, failure

15 September 2018 6,865 1 View

What is Multifractal IDW interpolation method? Is this a ordinary IDW interpolated layer reclassifed by the results obtained from a fractal method?

I came across this term in a paper by A Lima 2003. Please make me understand the basic concept and how can I apply it in continuous field.

17 June 2018 1,329 0 View

Does counterpoise correction not consider electron delocalization between two fragments if there is a possibility of electron delocalization ?

I want to calculate the complexation energy of two stacked aromatic system. If I use counterpoise correction then does it not consider non covalent electron delocalization (n to sigma* or pi to pi*) ?

02 January 2018 3,600 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View