How much is the role of education in living a happy life?

What is the purpose of education and what we get from education?

23 September 2020 5,949 4 View

What should be the standard glucose concentration to be used in high glucose studies, that match the physiological levels?

Forgive me for being Naive. I have read that the postprandial concentration normally doesn't go above 140mg/dl, anything above it is considered diabetic. for instance:...

23 July 2019 1,969 4 View

What could be the possible reasons behind the cells dying in the culture plate in few wells while surviving in other, media conditions being same?

I work on MC3T3-E1 cell lines. The cells appear absolutely fine in flasks, but when I seed them in 12/24 well plates for mineralization (nunc thermofischer plates), then cells appear to die in few...

03 February 2019 1,648 3 View

How to extract particular section from text data using NLP in Python?

I have lot of CV (text documents). From these data I want to extract particular section of 'Education Qualification', 'Experience', etc.

03 January 2019 4,454 12 View

Is Nested PCR approach to detect HPV infection in fresh as well as FFPE specimens right?

Hi! I am using nested PCR approach to detect mucosal HPV in cervical specimens as well as from FFPE tissues. This is to increase the sensitivity. But in doing so, I am getting a same similar size...

28 March 2018 9,631 4 View

How to prepare drug library?

There is no known inhibitor for my protein, so i want to screen some drugs which are natural compounds and most probably commercially available, so for the drug library, which database i used to...

03 October 2017 505 2 View

Pyruvate kinase M2 assay?

We have synthesized some inhibitors for PKM2 enzyme. we are planning to perform enzyme kinetic studies. we studied some protocols for the same but conc. of enzymes to be used are missing. some of...

18 September 2017 433 4 View

PLKO1 vectors transduction?

We are going to use SHC001 Scramble vector from sigma it does not contain any shRNA or GFP tag, however, it has ampicillin and puromycin resistance genes. In this case, how to characterize that...

12 September 2017 7,703 6 View

Which form of sh RNA is best for experimental purposes?

20 July 2017 4,414 3 View

Cancer stem cell isolation protocol?

Hello, I am isolation cancer stem cells from MCF-7 cells. I saw protocols for 96 well plate where 1 cell per well is seeded. How many cells should be seeded in 6 well plate.

29 March 2017 5,174 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View