17 Questions 8 Answers 0 Followers
Questions related from Heena Jariyal
We have synthesized some inhibitors for PKM2 enzyme. we are planning to perform enzyme kinetic studies. we studied some protocols for the same but conc. of enzymes to be used are missing. some of...
19 September 2017 570 4 View
We are going to use SHC001 Scramble vector from sigma it does not contain any shRNA or GFP tag, however, it has ampicillin and puromycin resistance genes. In this case, how to characterize that...
13 September 2017 7,827 6 View
I was curious to know what is the effect of heterozygous deletions on housekeeping genes. Do they affect their function like tumor suppressor or as in case of oncogenes? is there a reference to...
05 September 2017 3,878 1 View
We are planning to buy shRNA for our experiments. can anyone suggest which form is good like whether one should buy bacterial stocks, plasmid or lentiviral particles? Is it good to buy bacterial...
21 July 2017 4,562 3 View
When seen under microscope how to differentiate between cell clumps and tumerspheres. How to count them?
04 July 2017 3,334 1 View
Hello, We want to perform Cytochrome c oxidase assay to check electron transport chain function in our treated cells. Is it necessary to isolate mitochondria for the assay if yes why? Further, we...
15 June 2017 8,968 3 View
Hi, is there any accurate protocol for single cell seeding? please suggest.
03 June 2017 7,341 3 View
Is there a protocol for determining intracellular ATP concentrations without using commercial kits. Please suggest.
17 May 2017 477 1 View
I am working with MDAMB134VI. I need some images and cell specific markers of this cell line for reference. If someone have it please refer or suggest.
19 April 2017 5,757 1 View
Hello, I am isolation cancer stem cells from MCF-7 cells. I saw protocols for 96 well plate where 1 cell per well is seeded. How many cells should be seeded in 6 well plate.
30 March 2017 5,327 3 View
Hello, I wonder if we want to study a gene silencing in Cancer stem cells and we are doing a Knockdown in cancer cells. After a stable knockdown if we isolate cancer stem cells from same cell...
14 March 2017 3,514 2 View
Hello, we are working with a specific peptide and want to check whether it is stable in particular formulation or not. Is there a change in PI of peptides when there conformation is disturbed and...
02 March 2017 9,309 6 View
hello, My cell line is having round morphology but i can see some cells in culture which are having different morphology . These spindle shaped cells are not growing fast how can I remove these...
23 January 2017 3,641 3 View
We want to work human dermal fibroblast cells from ATCC (ATCC® PCS-201-012™). It is recommended by ATCC to grow these cells in fibroblast basal medium supplemented with some growth factors....
30 December 2016 181 5 View
I am working with MCF7 cell lines and want to check expression of Glutaminase 2 ( GLS2) . it is reported in one article that the cells have GLS2 expression. I had tried it at different annealing...
27 December 2016 5,777 1 View
we use FBS and 10% DMSO as cryo media for preservation of mammalian cells . how long it can be stored or used and is their any special condition for storage? please suggest..
26 December 2016 4,138 1 View
we procured Adipose derived Mesenchymal stem cells from himedia and cryo preserved them. when we are reviving these cells they grow good for 3 to 4 days and then black dots appear in the flask but...
26 December 2016 8,463 4 View
