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Questions related from Heena Jariyal
We have synthesized some inhibitors for PKM2 enzyme. we are planning to perform enzyme kinetic studies. we studied some protocols for the same but conc. of enzymes to be used are missing. some of...
19 September 2017 491 4 View
We are going to use SHC001 Scramble vector from sigma it does not contain any shRNA or GFP tag, however, it has ampicillin and puromycin resistance genes. In this case, how to characterize that...
13 September 2017 7,765 6 View
We are planning to buy shRNA for our experiments. can anyone suggest which form is good like whether one should buy bacterial stocks, plasmid or lentiviral particles? Is it good to buy bacterial...
21 July 2017 4,480 3 View
Hi, is there any accurate protocol for single cell seeding? please suggest.
03 June 2017 7,276 3 View
Is there a protocol for determining intracellular ATP concentrations without using commercial kits. Please suggest.
17 May 2017 413 1 View
Hello, I am isolation cancer stem cells from MCF-7 cells. I saw protocols for 96 well plate where 1 cell per well is seeded. How many cells should be seeded in 6 well plate.
30 March 2017 5,241 3 View
Hello, I wonder if we want to study a gene silencing in Cancer stem cells and we are doing a Knockdown in cancer cells. After a stable knockdown if we isolate cancer stem cells from same cell...
14 March 2017 3,443 2 View
I am working with MCF7 cell lines and want to check expression of Glutaminase 2 ( GLS2) . it is reported in one article that the cells have GLS2 expression. I had tried it at different annealing...
27 December 2016 5,728 1 View
we use FBS and 10% DMSO as cryo media for preservation of mammalian cells . how long it can be stored or used and is their any special condition for storage? please suggest..
26 December 2016 4,066 1 View
