Hi All, I have several trials for few months to extract good genomic DNA from some cultures of Asp. nidulans, most of them worked with CTAB extraction from freez-dried mycelium (30-40-60 hrs old) except one isolate fails every time, every time I have good string DNA and clear white pellet which is hardly soluble in TE, but partially soluble after warming for hour at 65 C. Finally I have low concentrations of DNA on Qubit/Nanodrop but nothing appear on Gel!!
Any suggestion/ keys would be helpful for extraction?
Where is my DNA gone , What is the pellet if not a DNA??
From Aspergillus that I can get to sporulate, I tend to extract DNA from spores. It gives me a cleaner solution in the end - not as much protein.
This is my protocol, feel free to compare with yours and use:
https://benchling.com/s/prt-jkSV97rE8kY2MeZ6Gi9b
Hoda M El-Gharabawy I am attaching herewith the method we have successfully used for isolation of genomic DNA from filamentous fungi like Aspergillus sp., Metarhizium anisopliae, Mucor sp., and Benjaminialla poitrasii. Follow this, you would get the better quality DNA preparation of higher concentration.
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