I am attempting to amplify a small amount of genomic DNA into a much higher quantity for future experiments using random hexamers (NNN NNN) as the primer set. Currently, I am running this protocol with standard initial denaturation and final extensions for the master mix that I am using. Furthermore, the cycling step involved denaturation as per the master mix instructions, an annealing step of 10°C for 30 seconds, and an extension at 72°C for one minute, with this cycle repeating for 40 cycles. This was a modified PCR protocol from previous experiments and has not shown to amplify the DNA. What are some ways I can change or add onto this protocol? Furthermore, if you have conducted similar experiments that have worked in the past, would you be able to provide the protocol that you used?