How to extract DNA from Rovers Orcellex Brush?

Hello researchers I have a fresh oral tissue samples collected by Rovers Orcellex Brush , unfortunately I can not use any kit. Please, I need trusted protocol to extract DNA from this brush...

10 November 2019 8,270 3 View

What is the primers sequences of SNORD44 ?

Hello every one I'm working on micRNA expression and need the SNORD44 for normalization , please i need the sequences of SNORD44 primer because i cant find it in the published papers...

04 May 2019 4,751 1 View

What is the reasonable explanation of this abnormal haplotypes association odd ratio?

Hello everyone I'm working on haplotypes case control association study, for data analysis , I employed two online analysis softwares , The first is (SNPstats) which gave me extremely large odd...

11 December 2018 1,308 1 View

Why there are two different protocols to isolate DNA from frozen and fresh blood ?

Hello researchers all of us know, that we need a special protocol to extract DNA from frozen blood other than protocol which used to extract DNA from fresh blood . the question is : .what are...

08 September 2018 311 5 View

What are the detailed buffer recipes and protocol to isolate and purify genomic DNA by silica spin column ?

Hello researchers Can somebody please , provide me the recipes to prepare buffers that used with silica spin column and the protocol to isolate the genomic DNA from blood . best regards

02 March 2018 1,065 4 View

How to estimate RNA polymerase II activity in eukaryotes?

Hello researchers . one of my colleagues develops a new drug and he think that this drug inhibit the activity of RNA polymerase II (transcription ) . the question is , how to estimate this...

01 February 2018 7,574 2 View

Exonic or intronc region, which one is more potential to has polymorphisms that are associated with a trait ?

Hello researches I'm planning to sequence about 800 bp region of a certain gene to assess its association with a certain disease. the question is , do i have to choose an intronc or exonic...

31 December 2017 3,300 5 View

In HPLC/UV , can the negative peak resulted from fluorescent material?

Hello researchersthere are several reasons to observe a negative peak in HPLC/UV chromatogram , but can this negative peak be due to the presence of fluorescent material. best regards ....

08 September 2017 6,391 4 View

Annealing time or annealing temperature which one is better for PCR optimization ? and why?

Hello researchers the annealing temperature gradient is the routine step in PCR optimization , but why we do not use annealing time for optimization? regards .

07 August 2017 5,874 15 View

Is it essential to use high fidelity-pcr prior to sequencing ?

06 July 2017 3,748 9 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View