You might need to generate neurospheres first by using neural basal media with subsequent use of neural differentiation media.

Hello

This articles may help you

Good Luck

http://onlinelibrary.wiley.com/doi/10.1002/stem.1174/pdf

http://journal-inflammation.biomedcentral.com/articles/10.1186/1476-9255-9-12

Hi Saleh,

I've seen that article (and another from that group). However, they were not using mesenchymal stem cells.

Thank you though!

Dear Lauren

It is very interesting question "Generation of microglia from human mesenchymal stem cells?"

But I have some question.

1- Why microglia from MSCs not from HSC (hematopoetic stem cells).

Hinze A, Stolzing A. Microglia differentiation using a culture system for the expansion of mice non-adherent bone marrow stem cells. Journal of inflammation. 2012 Apr 02;9(1):12. PubMed PMID: 22471998. Pubmed Central PMCID: 3495406.

Muffat J, Li Y, Yuan B, Mitalipova M, Omer A, Corcoran S, et al. Efficient derivation of microglia-like cells from human pluripotent stem cells. Nature medicine. 2016 Nov;22(11):1358-67. PubMed PMID: 27668937. Pubmed Central PMCID: 5101156.

Hinze A, Stolzing A. Differentiation of mouse bone marrow derived stem cells toward microglia-like cells. BMC cell biology. 2011 Aug 19;12:35. PubMed PMID: 21854582. Pubmed Central PMCID: 3175184.

The references may help you .

2- Microglia belongs to hematopietic lineage

HSCs------myeloid progenitor cells- -----yold sac microglia cell progenitor-------microglia. 

I SUGGEST YOU TO DIFFERENTIATE MSCS TO MYELOID PROGENITOR CELLS THEN IT IS VERY EASY TO DIFFERENTIATE MYELLID PROGENITOR TO MICROGLIA CELLS