Lauren S Sherman

10 Questions 20 Answers 0 Followers

Questions related from Lauren S Sherman

Effect of flash freezing of pelleted cells?

EDIT: I KNOW IT ultimately RESULTS IN CELL LYSIS. My question is how? Is it just crystal formation? It seems to me that snap freezing the cells results in a sensitization of sorts of the cell...

21 February 2017 8,042 2 View

Generation of microglia from human mesenchymal stem cells?

I am seeking a method to generate microglia from human mesenchymal stem cells (preferably bone marrow derived MSCs). Does anyone have a method to suggest?

24 May 2016 5,115 4 View

One-way MLR: optimal dose for irradiating PBMCs?

I'm looking for the lowest possible dose of gamma radiation needed to inhibit proliferation of my stimulator PBMCs in a one-way MLR. I don't want to over-irradiate and damage the MHC molecules or...

06 November 2015 584 2 View

Looking for a glycosylation stain

I am looking for a stain to detect glycosylations. Does anyone have any suggestions for a *mass spec safe* kit or homemade protocol? I do not know how much glycosylation will be in a given band,...

06 June 2013 1,297 4 View

Are there any post translational modifications that include addition of a thiol?

I know there are PTMs that make use of the thiol group on cysteine, but does anyone know of any PTMs that add a thiol group to a protein (in any organism, not just humans)?

19 March 2013 5,720 1 View

What controls are needed in immunoprecipitation?

I have a custom antibody (it works for Western blot and immunohistochemistry) that I would like to try for immunoprecipitation. I know that the antibody successfully binds my beads (I'm...

18 March 2013 3,617 6 View

Good tool for qRTPCR primer design?

I'm designing some primers for qRTPCR. Does anyone have any suggestions for a good qRTPCR primer design tool? (I expect to need to check for hairpins and dimers and what not, but would prefer...

13 September 2012 5,669 5 View

How to evaluate EDU in cells that are non-adherent without flow cytometry?

We are studying cell proliferation in a cell type (from a marine invertebrate) that is non-adherent. Does anyone have suggestions for method(s) to evaluate EDU labeling in non adherent cells?...

31 August 2012 3,370 1 View

Why won't SDS stay in solution? (SDS/Tris solution)

I'm making a stock of O'Farrell solution for SDS-PAGE gel casting. The recipe calls for 1.5M Tris pH 8.8 and 0.4% SDS in water, final pH 8.8. Each time I or a lab-mate try making this solution,...

15 May 2012 6,440 8 View

How can I desalt a protein sample currently in >300mM salt?

I have a protein family with a histidine rich region that I am successfully isolating by metal affinity chromatography. I have previously run these samples on one-dimensional SDS-PAGE gels...

04 May 2012 8,485 33 View