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Questions related from Lauren S Sherman
EDIT: I KNOW IT ultimately RESULTS IN CELL LYSIS. My question is how? Is it just crystal formation? It seems to me that snap freezing the cells results in a sensitization of sorts of the cell...
21 February 2017 7,992 2 View
I'm looking for the lowest possible dose of gamma radiation needed to inhibit proliferation of my stimulator PBMCs in a one-way MLR. I don't want to over-irradiate and damage the MHC molecules or...
06 November 2015 535 2 View
I know there are PTMs that make use of the thiol group on cysteine, but does anyone know of any PTMs that add a thiol group to a protein (in any organism, not just humans)?
19 March 2013 5,681 1 View
I have a custom antibody (it works for Western blot and immunohistochemistry) that I would like to try for immunoprecipitation. I know that the antibody successfully binds my beads (I'm...
18 March 2013 3,566 6 View
I'm designing some primers for qRTPCR. Does anyone have any suggestions for a good qRTPCR primer design tool? (I expect to need to check for hairpins and dimers and what not, but would prefer...
13 September 2012 5,626 5 View
We are studying cell proliferation in a cell type (from a marine invertebrate) that is non-adherent. Does anyone have suggestions for method(s) to evaluate EDU labeling in non adherent cells?...
31 August 2012 3,327 1 View
I'm making a stock of O'Farrell solution for SDS-PAGE gel casting. The recipe calls for 1.5M Tris pH 8.8 and 0.4% SDS in water, final pH 8.8. Each time I or a lab-mate try making this solution,...
15 May 2012 6,392 8 View
I have a protein family with a histidine rich region that I am successfully isolating by metal affinity chromatography. I have previously run these samples on one-dimensional SDS-PAGE gels...
04 May 2012 8,427 33 View
