NUTRIENT MEDIA FOR OBTAINING A PURE CULTURE OF FUNGI OF THE GENUS Pleurotus in vitro
April 2016
DOI: 10.15407/biotech9.02.082
LicenseCC BY 4.0
T. V. Ivanova
Which species do you use? And do you autoclave the malt? Do you make maltextract-media or do you use pure malt?
A lot of plant pathologists have used malt agar but it certainly is not as popular as potato dextrose agar which is most commonly employed. Many Pleurotus species grow well on straw and seeds. Inoculum can be increased on seeds of millet which will give many points of colonization of commercial oyster mushroom cultures. To get cleanest oyster cultures the isolation can be in the fresh young stipes where outer parts are disinfected and the center stipe is picked using aseptic technique under clean hood facility.
Would not expect growth of pure malt [extract?] - osmotic potential far too high. Aseptic removal of small pieces of tissue with flamed needle from the central stem tissue of developing sporophores on to Potato Dextrose Agar should work. Examine colony edge daily and subculture from colony edge if any contamination is seen.
Use 100 g of potatoes which are cubed and boiled. Strain off the solids. Dissolve 1 to 2% as dextrose 10 to 20 grams for a liter. Dissolve 1.5% 15 g of agar. Dilute to 1000 ml one Liter autoclave from 20 minutes at 121 C. Cool and serve under aseptic condition into sterilized petri plates.
I have included a simple effective recipe for potato dextrose agar for propagating your oyster mushroom. Michael Richardson comments are very good and get the basic manipulation.
You can buy PDA from lab. suppliers - Fisher, Sigma Aldrich etc. - Google local suppliers - it should not be too expensive
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