O'Donnell & Beneke (1973) use two culture media to grow Peziza quelepidotia Korf & O'Donnell , YMA and JPA. YMA consists of 3 g of yeast extract, 3 g of malt extract, 5 g of peptone, 10 g of glucose and 20 g of agar per L. On the other hand, the JPA medium consists of 3 Jiffy-7® homogenized pellets plus 20 g of agar per L. The cultures were performed at 24-25 ° C under continuous fluorescent illumination. The fungus is capable of producing apothecia in JPA medium.

Article Apothecial Formation by Peziza Quelepidotia in Pure Culture

No, I am not culturing Peziza sp.

I would start with methods used for related Ascobolus. It was genetic model (1950s-1970s) and cultured on defined media to score metabolic mutants.

O'Donnell & Beneke (1973) use two culture media to grow Peziza quelepidotia Korf & O'Donnell , YMA and JPA. YMA consists of 3 g of yeast extract, 3 g of malt extract, 5 g of peptone, 10 g of glucose and 20 g of agar per L. On the other hand, the JPA medium consists of 3 Jiffy-7® homogenized pellets plus 20 g of agar per L. The cultures were performed at 24-25 ° C under continuous fluorescent illumination. The fungus is capable of producing apothecia in JPA medium.

Article Apothecial Formation by Peziza Quelepidotia in Pure Culture

The peziza the mycelial growth only observed in YMG,CMA medium not produced any apothecia .

Any photo for the pure culture plz ??

Sridhar Pichai Marc Stadler Cristian Riquelme Stephen M Marek