What could be the possible explanation that a gene is present in two parents but not expressed?

What could be the possible explanation that a gene is present in two parents but not expressed. However it is expressed in the F1hybrid. Does it have any connection with Ac/Ds transposition.

09 October 2017 2,366 0 View

How to isolate endophytic Bacteria from paotato?

Dear all Please send me a protocol to isolate endophytic bacillus species from potato, thanks.

02 March 2017 5,396 18 View

How will i select the signal peptide for my gene expression?

Dear All If i have a gene sequence and i wanna to express it in E. coli, how will i know the sutaible signal peptide which could help in expressing the gene? The gene sequence was found through MS...

07 August 2016 6,566 8 View

How can i desalt my protein sample, if i do not have dialysis Tube?

Hi Dear all Is there some other ways to desalt my crude protein sample, if i dont have dialysis tubes, The proteins are precipitated with 80% amonium salphate. I do not wanna to loss even 4kDa...

07 August 2016 5,168 7 View

How to find the identicle peptide sequences through MASCOT data base?

Dear All I have three peptides sequences from MALDI TOF/TOF MS analysis, now i am confused where to blast in MASCOT database and the second confusion is that i have to separately blast each one or...

07 August 2016 4,074 6 View

What is the procedure for crude protein precipitation with ammonium sulfate from culture filtrate of the fungal strain?

i want to purify elicitor proteins from the crude proteins of cuture filterate of fungi, therefore, i need to precipitate (80% saturation) the crude proteins.

05 June 2016 3,975 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View