What could be the possible explanation that a gene is present in two parents but not expressed. However it is expressed in the F1hybrid. Does it have any connection with Ac/Ds transposition.
Dear all Please send me a protocol to isolate endophytic bacillus species from potato, thanks.
02 March 2017 5,484 18 View
Dear All Could anyone help me guide for cloning and expression of heterologous protein gene in pP43NMK vector. As the pP43NMK has P43 (promotor), i need to insert SP on N-terminal of P43. So from...
08 September 2016 4,797 0 View
Dear All If i have a gene sequence and i wanna to express it in E. coli, how will i know the sutaible signal peptide which could help in expressing the gene? The gene sequence was found through MS...
07 August 2016 6,658 8 View
Hi Dear all Is there some other ways to desalt my crude protein sample, if i dont have dialysis tubes, The proteins are precipitated with 80% amonium salphate. I do not wanna to loss even 4kDa...
07 August 2016 5,296 7 View
Dear All I have three peptides sequences from MALDI TOF/TOF MS analysis, now i am confused where to blast in MASCOT database and the second confusion is that i have to separately blast each one or...
07 August 2016 4,195 6 View
i want to purify elicitor proteins from the crude proteins of cuture filterate of fungi, therefore, i need to precipitate (80% saturation) the crude proteins.
05 June 2016 4,086 1 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this
05 August 2024 7,902 3 View
I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...
02 August 2024 2,627 1 View
How can parents ensure their drinking water is free from harmful contaminants?
27 July 2024 8,722 6 View
I want to know that n-doped side of solar cell is connected with positive or negative electrode.
20 July 2024 1,348 2 View
I studying PhD course in Urban design for Health. I need to know in some fileds: residental block, green open space and Transport TOD. I need connect with expert in that fileds.
14 July 2024 1,892 3 View
All I want to know is whether it is feasible or not. How did genes come to be if they do not come from parents?
12 July 2024 7,221 1 View
Enhancing parental satisfaction through the comprehensive debriefing of fetal anomalies using 3D models- this is my research topic. I kindly request to suggest some questions that can be asked to...
06 July 2024 8,032 0 View
"Today several adjectival phrases have been used to describe English like ‘International Language’, ‘Lingua-Franca’, ‘Language for Globally Connecting’, ‘Library Language’, ‘Official Language’,...
01 July 2024 8,406 5 View
I want to know whose mistake or fault it is that I have to suffer in this world which I didn't choose or decide to live? Is it my parent's fault or their parent's parent's fault?
01 July 2024 3,262 2 View