What could be the possible explanation that a gene is present in two parents but not expressed. However it is expressed in the F1hybrid. Does it have any connection with Ac/Ds transposition.
Dear all Please send me a protocol to isolate endophytic bacillus species from potato, thanks.
02 March 2017 5,396 18 View
Dear All Could anyone help me guide for cloning and expression of heterologous protein gene in pP43NMK vector. As the pP43NMK has P43 (promotor), i need to insert SP on N-terminal of P43. So from...
08 September 2016 4,702 0 View
Dear All If i have a gene sequence and i wanna to express it in E. coli, how will i know the sutaible signal peptide which could help in expressing the gene? The gene sequence was found through MS...
07 August 2016 6,566 8 View
Hi Dear all Is there some other ways to desalt my crude protein sample, if i dont have dialysis tubes, The proteins are precipitated with 80% amonium salphate. I do not wanna to loss even 4kDa...
07 August 2016 5,168 7 View
Dear All I have three peptides sequences from MALDI TOF/TOF MS analysis, now i am confused where to blast in MASCOT database and the second confusion is that i have to separately blast each one or...
07 August 2016 4,074 6 View
i want to purify elicitor proteins from the crude proteins of cuture filterate of fungi, therefore, i need to precipitate (80% saturation) the crude proteins.
05 June 2016 3,975 1 View
Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...
28 February 2021 2,116 1 View
I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...
27 February 2021 6,818 3 View
I am interested in evaluating mesh optimization solvers that converge to high aspect ratio unstructured hexahedral grids. In other words, I am interested in a solver that optimizes warpage,...
26 February 2021 7,279 2 View
I am trying to seek out some Transgenomic Wavemaker software (happy to purchase a computer too) to operate a fraction collector. The only way to connect to the fraction collector so far is to...
23 February 2021 9,181 1 View
I am working with several continous, environmental marine datasets at varying original spatial resolutions. My aim is to regrid each dataset to one common, high resolution grid. I am currently...
22 February 2021 2,290 2 View
Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...
21 February 2021 2,247 3 View
Dear colleagues! We have prepared an article on the current status of human resources potential of municipal state forest management institutions of Kazakhstan. Since this is my first experience...
16 February 2021 8,611 3 View
Greetings, I was wondering what is the optimal way to connect the reinforcement at the top of the pile (the part that connects to the raft foundation): -The pile bars are left straight and...
16 February 2021 2,007 3 View
Is there any kind of data available for connection strength between UE and RAN? I am looking for signal strength, the geographical coverage of RAN's in a given Tracking Area, Coverage Area over a...
16 February 2021 1,073 5 View
I am designing a DAB DC DC Converter and when I am connecting a battery block in Matlab and fix its specification lets say 350 V and 25 A, when I am giving input as 600V, Battery would charge to...
11 February 2021 3,110 1 View