Cell pellet becomes sticky / insoluble in nuclear extraction buffer?

24 May 2016 8,013 2 View

Cell pellet becomes sticky / insoluble in nuclear extraction buffer?

04 May 2016 5,180 3 View

The meaning of X and the preparetion of X solution?

02 March 2016 2,506 2 View

Pre-run EMSA gel (non-denaturing gel) but not for SDS-PAGE?

Why don't we pre-run (or equilibrate) the SDS-PAGE like how we do for EMSA gel?

01 February 2016 9,448 4 View

Why does a stacking gel leak?

After I pouring resolving gel, I put a layer of isopropanol. Resolving gel looked polymerised well. However, after I pouring stacking gel on top of it and letting it to be polymerised, I found...

03 January 2016 3,368 7 View

Why does a stacking gel leak?

After I pouring resolving gel, I put a layer of isopropanol. Resolving gel looked polymerised well. However, after I pouring stacking gel on top of it and letting it to be polymerised, I found...

31 December 2015 7,316 7 View

How do I guarantee the number of cells for western blot or other experiments?

When I read through the method of some journals, they will mention the number of cells they used, for example 1x10^6 cells for immunoblotting. My question is how do we ensure that there is 1x10^6...

16 December 2015 2,621 4 View

Why does ethanol not affect the absorbance of Bradford assay?

11 December 2015 5,338 7 View

Spit ratio for cell tissue culture?

An old and stupid questions for all the newer of cell culture.. I found that there are two definitions of split ratio on the web: 1. 1:7 = 1ml of cell resuspended + 9ml of medium 2. 1:7 = 1ml from...

11 December 2015 8,287 5 View

How do I guarantee the number of cells for western blot or other experiments?

When I read through the method of some journals, they will mention the number of cells they used, for example 1x10^6 cells for immunoblotting. My question is how do we ensure that there is 1x10^6...

11 December 2015 6,390 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View