Free-floating IHC - What is the function of 0.1 acetate buffer?

22 November 2023 0 9K Report

I'm checking free-floating IHC protocol for my research using mouse brain sample.

I've never performed free-floating IHC before, so I am checking protocol deeply.

In protocol during several website, it is mentioned that "Wash the tissue 0.1M acetate buffer after incubating 2nd antibody".

Is there any reasons about using 0.1M acetate buffer instead of PBS during washing step?

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