Its SImple

Tm = 2 X (A+T) + 4 X (G+C)

it will give you approx tm of your primers otherwise there are many tm calculator available online.

http://www6.appliedbiosystems.com/support/techtools/calc/

https://www.neb.com/tools-and-resources/interactive-tools/tm-calculator

http://www.promega.com/techserv/tools/biomath/calc11.htm

http://www.thermoscientificbio.com/webtools/tmc/

Hi Bhairavi,

You can use various sites for your Tm calculation as:

www6.appliedbiosystems.com/support/techtools/calc/

https://www.neb.com/tools-and-resources/interactive.../tm-calculator

www.promega.com/biomath/calc11.htm‎

Two standard approximation calculations are used.

For sequences less than 14 nucleotides the formula is:

Tm= (wA+xT) * 2 + (yG+zC) * 4

where w,x,y,z are the number of the bases A,T,G,C in the sequence, respectively.

For sequences longer than 13 nucleotides, the equation used is

Tm= 64.9 +41*(yG+zC-16.4)/(wA+xT+yG+zC)

When degenerated nucleotides are included in the primer sequence (Y,R,W,S,K,M,D,V,H,B or N), those nucleotides will be internally substituted prior to minimum and maximum Tm calculation.

Its SImple

Tm = 2 X (A+T) + 4 X (G+C)

it will give you approx tm of your primers otherwise there are many tm calculator available online.

http://www6.appliedbiosystems.com/support/techtools/calc/

https://www.neb.com/tools-and-resources/interactive-tools/tm-calculator

http://www.promega.com/techserv/tools/biomath/calc11.htm

http://www.thermoscientificbio.com/webtools/tmc/

I think it would be helpful for youhttp://nar.oxfordjournals.org/content/31/13/3406.abstract

This works better (In my optimization) Tm = 2 X (A+T) + 4 X (G+C) - 2

Hello, I think that this site can help you, there are three ways to calculate the TM, according to your interests.

http://www.basic.northwestern.edu/biotools/OligoCalc.html

It is also possible to use a desktop program pDRAW32 by downloading it to your PC.

You can study this link: http://www.youtube.com/watch?v=84FlXQ6fxMI.

Just enter your primer sequences. it is enough...

Tm = 2 X (A+T) + 4 X (G+C)

Hi

You can try this website

https://www.biophp.org/minitools/melting_temperature/demo.php?formula=basic

Hello, the most accurate algorithms for Tm prediction are based on the nearest neighbor method. You can use the following online application:

http://www.molbiotools.com/primerinspector.php

The current formula is for one primer, what about the two primers together (reverse and forward).

Hello Nuha, the Primer Inspector app (see the link above) allows you to analyze any number of primers you need all at once.