Babak Nami

4 Questions 37 Answers 0 Followers

Questions related from Babak Nami

Are Fluorescein/FITC (as donor) and mCherry (as acceptor) good FRET pairs?

Hi colleagues, I am planning to study the interaction of a DNA-binding protein with a plasmid DNA sequence by FRET assay in vivo. I want to label my plasmid with FITC and transfect it into...

20 February 2023 7,990 4 View

Does bacteriophage (T2, T4,..) genome works in human cells?

I want to transfect phage T2 genome into HEK293 cells in order to produce phage particles and cell lysis. But I don't know whether the genes will work or not. What is phage biology in mammalian cells?

07 October 2013 3,027 1 View

Why do circular DNAs (plasmid, BAC, ...) make a smear during running in agarose gel?

Circular DNA makes a smear when running in agarose gel, but the digested linear form doesn't. I am not talking about covalently or supercoiled bonded.

03 July 2013 6,189 10 View

What is this kind of morphology?

These are AGS cells (Gastric cancer cell line) after incubation in a low pH stale RPMI + 1% L-glutamine +10% FBS + 2% antibiotic for 10 days without medium refresh. When I check the cells most...

18 June 2013 2,135 7 View