Not all introns have the GU-AG boundary, but a special class of genes. Perhaps this link can help you: http://www.ncbi.nlm.nih.gov/books/NBK21132/ (chapter 10.1.3 - 'Intron splicing')
Not all introns have the GU-AG boundary, but a special class of genes. Perhaps this link can help you: http://www.ncbi.nlm.nih.gov/books/NBK21132/ (chapter 10.1.3 - 'Intron splicing')
There are more sequences involved in the consensus splice sites. http://www.life.umd.edu/labs/mount/RNAinfo/consensus.html
The spliceosome need some sequences to do correct splicing such as AG/GT in exon- intron boundaries, A branch and ...
The spliceosome recognize the AG-GT sequence on the exon-intron junction, to splce out the intron. If there is mutation in the splice site junction, it leads to the exon skipping and Intron retention in the mRNA transcript.
We would reccomment to use this website for splicing tools:
http://astlab.tau.ac.il/index.php
We have used the website suggested by Qing-Rong Liu for a publication to Human Mutation, and they did not agree with this server and reccommended the first one.
I think to directly answer your questions, the splicing complex incorporate U1 and U2 snRNA and they recognize splice site by base-pairing, GT pairs with U1's AC, as U1 release, U2's CU pairing to intron 3''s AG.
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