You want to isolate by Kit method or manual method?

Hi Bhairavi, I am assuming you are looking for free DNA or cells from the baby?

For free DNA or for cells in the body fluid, treat it like blood- I would try UC Blood Spin DNA Kit from MO BIO Laboratories. http://www.mobio.com/blood-dna-isolation/ultraclean-bloodspin-dna-isolation-kit-1.html

You can get a free sample of the kit to test it.

Hi Bhairavi,

Here is the answer from our Technical Support Person at MO BIO:

I'm not sure what volume they would be starting with. However, I'd suggest that they centrifuge the liquid at 10,000 x g for 2 minutes to pellet the cells. They would then remove the liquid and start with this cell pellet.

If there are low levels of inhibitors and they want microbial DNA they could use the UltraClean Microbial kit. If the liquid is contaminated by blood, meconium etc. they could use the BiOstic Bacteremia kit starting from the cell pellet. This would remove inhibitors.

If they are looking for human DNA from cells and there are low levels of inhibitors, I'd suggest the UltraClean Tissue & Cells Isolation kit starting with the cell pellet, following the protocol for cultured cells.

If they want human cells and the fluid is contaminated then they could again use the BiOstic Bacteremia kit. But they would not use the bead tube. Instead they would start by resuspending the cell pellet in Solution CB1, vortex for 10 seconds to mix and place at 70 C for 15 minutes with 10 ul of Pro K. The rest of the protocol would be as usual.

I hope it helps.

Again we have samples of all the kits mentioned above if you feel like testing it.